LOCUS       sequence-139210-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Vo BT, Wolf E, Kawauchi D, Gebhardt A, Rehg JE, Finkelstein D, Walz 
            S, Murphy BL, Youn YH, Han YG, Eilers M, Roussel MF
  TITLE     The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup 
            Identity.
  JOURNAL   Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 
            10.1016/j.ccell.2015.12.003.
  PUBMED    26766587
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.12"; journalName: "Cancer Cell"; date: 
            "2016-01-11- 11"; volume: "29"; issue: "1"; pages: "5-16"
COMMENT     Sequence Label: pCDNA3-GFP
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    2..54
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(68..70,71..73,74..784)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(68..70,71..73,74..784)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(113..134)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(374..393)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     721..742
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccggt
       61 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      121 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      181 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      241 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      301 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      361 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      421 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      481 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      541 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      601 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      661 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      721 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      781 caagaagctt agccatggct tcccgccgg
//
