LOCUS       sequence-139223-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pTARBAC2 with GalK selection marker and 
            lens-specific transgenesis marker Citrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..710,711..713,714..716))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(42..63)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     391..410
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     650..671
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      713..722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
       61 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg
      121 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      181 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      241 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      301 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      361 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      421 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      481 atgtggtcgg ggtagcgggc gaagcacatc aggccgtagc cgaaggtggt cacgagggtg
      541 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      601 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      661 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
      721 gcgaccggtg gatccacagt acaaagatcc ccaaatgatg tgtgccggcc ttagtggagg
      781 cagacccctg gttatatacc tgtgctgcag aaagaggggc ttacaaatgg atctctctgg
      841 aatggcatta tctcatggtg ggaacagtgt tgtcagcaga atcagcgggg tggactctga
      901 cctgagaatg acagtctagc actgaat
//
