LOCUS       sequence-139232-        8573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinC1 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnc1-Fc-His
FEATURES             Location/Qualifiers
     source          1..8573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5153..5170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5196..5317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5233..5252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5287..5306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5355..5372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5526..6114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5606..5625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6285..7076,7077..7145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6908..6927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7146..7250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7318..7336
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7374..7396)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7398..7418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7403..7733
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7584..7719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7646..7665
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7870..7889
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7956..8411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8093..8114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8305..8324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8537..8559
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8551..8568
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8552..8568
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggaggtctc
     1621 ccggaggaag acgccgccgc gccccccgta ccccgccgca ccgttgcccc tgatcgccta
     1681 tctgctggcg ctggcagcgc ccgcccgggg cgcggacgag ccggtgtggc gatctgagca
     1741 agccatcgga gccatcgcgg cgagccgggc ggacggcgtg ttcgtggcga gcggcagctg
     1801 cctggaccag ctggactaca gcctgaagaa caggctctct cgcctatacc gggaccaagc
     1861 gggcaactgc acggagcccg tctcgttggc gccccccgcg cggccccggc ccgggagcag
     1921 cttcagcaag ctactgctgc cctaccgcga gggggcgacc ggcctcgagg ggctgctgct
     1981 caccggctgg accttcgacc ggggcgcctg cgaggtgcgg cccctgggca acctgaaccg
     2041 cagctccctg cgcaacggca ccgaggtggt gtcatgtcac ccgcagggtt cgacggctgg
     2101 tgtggtgtac cgcgcgagtg gcaccgacct ctggtacctg gcggtggccg ccacctatgt
     2161 gctgccggag ccggagaccg cgaaccgctg caaccccgca gcatccgatc gcgacacggc
     2221 tatcgcgctc aagaacacgg aggggcgcag tctggccacg caggagctgg gccgcctgaa
     2281 actgcgcggt agtgcgggca gcctgcactt cgtggacgcc tttctctgga acggcagtgt
     2341 ctacttcccc tactatccct acaactacac gagtggcgct gccaccggct ggcccagcat
     2401 ggcgcgcatc gcgcagagca cagaggtgct gttccagggc caggctgccc tcgactgcga
     2461 ccacggccac cctgagggcc gccgcctact gctttcctcc agcctggtgg aggctgtgga
     2521 catctgggcc ggcgtgttca gcgcggccac tggagagggc caggagaggc gctcccccgc
     2581 caccacggct ctctgcctct tcaggatgag tgagatccag gcgcacgcca ggagctgcag
     2641 ctgggacttt caagcgactg agcacaactg caaagaaggg gatcggcctg aacgggtcca
     2701 gccaattgca tcgtctacac tgatccactc tgacctgacg tctgtttatg gcaccgtggt
     2761 gatgaacagg actgttctgt tcttggggac cggagatggc cagttactga aggttgttct
     2821 tggtgagaat ttgacttcaa actgtccaga ggttatttat gaaattaaag aagaaacgcc
     2881 tgtgttctac aagctcgttc ctcatcccat gaagaacatc tacatctacc taacagctgg
     2941 gaaggaggtg agaagaattc ctgttgcaaa ctgcagtaaa cggaaatcct gttcagagtg
     3001 cttagcagcg gcagaccccc actgtggctg gtgcctcccg ttacaaaggt gcacttttca
     3061 aggggattgc acacatgctg ggagctttga aaactggctg gatatttcat caggacctaa
     3121 aaaatgcccg aaaattcaga tacttcgaag cctccgagag agaaccacag tgaccattgt
     3181 gggaagcatc tccgcaagac actcggagtg cgtggtgaag aacgcagaca cgggcaagct
     3241 gctctgccag gggagaagcc aactgaactg gacctgtgcc tgcaatatcc ccagcagacc
     3301 cagctataat gtcttagttg tcaatgcgac cttctccttt ccgtcttgga acttatcaga
     3361 aagattcaac ttcaccaact gtgcgtcatt aaaagaatgc ccggcgtgta tcagaagcgg
     3421 ctgtgcttgg tgtaaacggg acaagaaatg catccacccc ttcaccccct gcgaaccatc
     3481 agactatgag aggaatcagg agctctgtca agttgctgtg gagaagtcgc ccaaggactc
     3541 aggaggagga agagtcaagg agagcaagag aaacagaact gacggggctg tgcaggtctt
     3601 ctacattaag gccattgagc cacaaaaaat atcaacctta gggaaaagca atgtgattgt
     3661 tactggagca aacttcaccc aggcgtcaaa tatcaccatg atcctgcgag gaaccagtac
     3721 ctgcgagagg gatgtgatcc gggttagcca tgtgctaaat gacacgcata tgaaattctc
     3781 acttccatca agccgcaaag aaatgaaaga cgtttgcatc cagtttgacg gtgggacctg
     3841 ctcgtctgca ggggctctgt cctacattgc tctgccacat tgttccctga tagttcctgc
     3901 caccacctgg atcagcggtg ggcaaaacat aaccatcatg ggcagaaatt ttgacgtcat
     3961 cgacaacctc atcatttcac acgagctgaa gggaaacgca aatgtgaaca taaatgtctc
     4021 tgaatactgc gcagcaactt tctgcaggtt tctagctcct aatttaaaga gctccaaagt
     4081 acgtacaaac gtggccgtga agctgagggt ccaagacacc tacttggact gtggaacgct
     4141 gcaatacctc gaggacccca gatttacagg gtaccgagtg gagtcagaga ttgatacaga
     4201 attggaagta aaaattcaaa aagaaaatga caatttcaac atttccaagg atgacattga
     4261 tattactctc ttccatgggg aaaataaaca atttaattgc agttttgaaa atattactag
     4321 aaaccaggat ctcacaacca tcctttgcaa gatcaagagt atcaagaatg caaacaccat
     4381 cgctagctcc tctaaaaaag tccgcgtcaa gctgggaaac ctggaactct atgtggaaca
     4441 ggaatcagtt ccttccggcg cgcctgacaa aactcacaca tgcccaccgt gcccagcacc
     4501 tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat
     4561 gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga
     4621 ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg
     4681 ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga
     4741 ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat
     4801 cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt ataccctgcc
     4861 cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt
     4921 ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa
     4981 gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt
     5041 ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct
     5101 gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaaggcg gccatcatca
     5161 ccatcaccat taatctagag atcttattaa agcagaactt gtttattgca gcttataatg
     5221 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5281 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg actctagcta
     5341 gactcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7261 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7321 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     7381 cacatgcagc tcccggagga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7441 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     7681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7741 cgggagcttg tatatccatt ttcgtcccgg agacggtcac agcttgtctg taagcggatg
     7801 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     7861 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     7921 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     7981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8401 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8461 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8521 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga att
//
