LOCUS       sequence-139248-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AacC2c1 human codon optimized protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ005 (AacC2c1 human codon optimized protein)
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      1028..1037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgggtcatca
       61 tcatcaccat cacaaaatcg aagaaggcaa actggttatt tggatcaatg gcgataaagg
      121 ctataatggt ctggcagaag ttggcaaaaa attcgaaaaa gataccggca ttaaagtgac
      181 cgttgaacat ccggataaac tggaagaaaa atttccgcag gttgcagcaa ccggtgatgg
      241 tccggatatt atcttttggg cacatgatcg ttttggtggt tatgcacaga gcggtctgct
      301 ggcagaaatt acaccggata aagcatttca ggacaaactg tatccgttta cctgggatgc
      361 agttcgctat aacggtaaac tgattgcata tccgattgca gttgaagcac tgagcctgat
      421 ctataacaaa gatctgctgc cgaatccgcc taaaacctgg gaagaaattc cggcactgga
      481 taaagaactg aaagcaaaag gtaaaagcgc actgatgttt aatctgcaag aaccgtattt
      541 tacctggcct ctgattgcag cagatggtgg ctatgcattc aaatatgaaa acggcaaata
      601 tgatatcaaa gacgtgggtg ttgataatgc gggtgcaaaa gccggtctga cctttctggt
      661 tgatctgatt aaaaacaaac acatgaacgc cgataccgat tatagcattg cagaagcagc
      721 atttaacaaa ggtgaaaccg caatgacaat taatggtccg tgggcatggt caaatattga
      781 taccagcaaa gtgaattatg gtgttaccgt tctgccgaca tttaaaggtc agccgagcaa
      841 accgtttgtt ggtgttctga gcgcaggtat taatgcagca agcccgaaca aagaactggc
      901 aaaagaattt ctggaaaact atctgctgac cgatgaaggt ctggaagcag tgaataaaga
      961 taaaccgctg ggtgcagttg cactgaaaag ctatgaagaa gaactggtta aagatccgcg
     1021 tattgcagcc accatggaaa
//
