LOCUS       sequence-139252-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AacC2c1 human codon optimized protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ005 (AacC2c1 human codon optimized protein)
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             101..121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gattaatgcc gcatcaggtc gtcagaccgt tgatgaagcc ctgaaagatg cccagaccgg
       61 tgcaagcacc ggatccctgg ttccgcgtgg tagcgctagc gaaaatctgt attttcaggg
      121 aatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccggtagtgc
      181 tgtgaagtct atcaaagtga agctccgcct ggatgacatg cccgagataa gggcagggct
      241 gtggaagctg cataaagagg ttaacgctgg cgtacggtac tacaccgaat ggctctcact
      301 tttgcggcag gaaaaccttt acagaagatc tcctaacgga gacggagaac aagagtgcga
      361 caagactgcg gaggaatgca aagcggagct gttggagagg cttagggccc ggcaggtgga
      421 gaatggccac aggggtcccg cgggcagcga tgatgagctg ctgcagcttg ctaggcagct
      481 ttatgagctg ttggttccgc aggcaatcgg ggcgaaaggc gatgctcagc agatcgcccg
      541 aaaattcttg tctccccttg ctgacaagga cgctgttggg ggtctgggta tagcgaaagc
      601 tggaaataag cccagatggg tgagaatgcg cgaagctggc gaacccgggt gggaagaaga
      661 aaaagagaag gcagagacta ggaaatccgc cgatcggaca gcagatgtac ttcgcgcact
      721 cgccgatttc gggctcaaac cactgatgcg ggtttacact gactcagaga tgtcctctgt
      781 cgaatggaaa cctttgcgca aaggccaggc tgttaggaca tgggaccggg acatgttcca
      841 gcaggcgatc gaaaggatga tgtcatggga gtcttggaac cagagagtcg ggcaggagta
      901 tgcaaaactg gttgaacaga agaaccggtt cgaacaaaag aattttgtcg gccaagagca
      961 tctggtgcac ctggtaaacc aattgcagca ggatatgaag gaggcctccc c
//
