LOCUS       sequence-139255-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AacC2c1 human codon optimized protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ005 (AacC2c1 human codon optimized protein)
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(69..113)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(114..137)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(162..242)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(249..296)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      899..908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgaca
       61 agcttttact cgtgccattc aattttctgg gcttcaaaga tatcgttcag gcctttatca
      121 tcatcatctt tataatcgct accaccaccc tcgaggcttt aggcatagtc ggggacatcg
      181 tatgggtatg cataatcagg cacgtcgtag ggataagcgt aatctggaac atcgtatggg
      241 taggatccct ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttcccg
      301 ctgccaatgt ctcctgtatt ttcgcaggcg ctgtcctgaa gggggacccg actccttatc
      361 tgtttcacca agtagccctc gatccgctga ttgaccatgg accaaaattc tttctggcgg
      421 gtccaattgc ctcggttaat gatcccagag ggatcgcgca tcagaacaac actcttctct
      481 cgggcttcat cggcctccac gaggagctcg gcttcctcct cgctcagctt ctcctgcgcg
      541 aacacctttc tcctcttctt tcccctttcc cgctcgtaat aagtaacgcc ggtattggta
      601 tagaacacct tgttagaata actgtctgct gtcctcttgc cggtcaacct tggaatcagc
      661 accagctcac catccacttc gccccaatca cagcgcaggc ggatctgaga gatgtcgaaa
      721 tcactccaga gcctctgctg caggttctga gcggcattca agtccgcgtg aatctggtga
      781 aagtctccct cttcggcgct aaaaggactc acaaatatct cgccctctcc ggttggaatc
      841 aggtcatcgg cgcgcagggg gcatgcgtcg agggtatgtt ccacaacgaa tttgttcagc
      901 caccatggga acggttcagg attatgttct tgagtacatc ttgcgggcac ccgtcgacac
      961 cgaatgccag gagcaccggt gcgtgcatca aa
//
