LOCUS       sequence-139260-        9431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA4 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna4-Fc-His
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6011..6028
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6054..6175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6091..6110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6145..6164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6213..6230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6384..6972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6464..6483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7143..7934,7935..8003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7766..7785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8004..8108)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8176..8194
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8232..8254)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8256..8276)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8261..8591
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8442..8577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8504..8523
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8728..8747
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8814..9269)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8951..8972)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9163..9182
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9395..9417
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9409..9426
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9410..9426
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgaaagccat
     1621 gccctggaac tggacttgcc tgctctccca cctgctggtg gtagggatgg gctcctccac
     1681 tctgcttcca cgacagccac cccagctgtc tcagaagcct tcctttgtga cattccgagg
     1741 ggagcctgct gagggcttca atcaccttgt ggtggatgag aggacagggc acatttattt
     1801 gggggctgtc aacagaatct acaaactctc tagtgacctc aaagtcttgg tgactcatca
     1861 gacagggcca gatgaggaca accccaagtg ttacccacct cggattgttc agacctgcaa
     1921 tgagcctctg gccagcacca ataacgtcaa caagatgctg cttatagact acaaagaaaa
     1981 caggctgatc gcgtgtggga gcctgtacca gggtatctgc aagctcctga ggctagagga
     2041 cctcttcaag ttgggagagc cctttcacaa gaaggaacat tatctctcgg gggttaacga
     2101 gagtggctca gtctttgggg tgattgtttc ctacagcaac tttgatgata aactctttat
     2161 tgccacagca gtggatggca aacctgaata ttttcctacc atctccagca ggaagctgac
     2221 taagaactct gaggccgatg gcatgtttgc ttacgttttc catgatgaat ttgtggcctc
     2281 tatgattaag atcccttcag acactttcac ggttatcccc gactttgaca tctactacgt
     2341 ctatggcttc agcagtggca attttgtcta ctttttgacc cttcagccag agatggtgtc
     2401 tcccccaggc tccacgacaa aggagcaggt gtacacctca aagcttgtga ggctttgcaa
     2461 agaggacaca gcctttaact cctacgtaga ggtgcccatt ggctgtgagc gcaatggggt
     2521 ggagtatcgc ttgttacagg ctgcctactt gtctaaagct ggtgcagtgc ttggccggac
     2581 cctaggagtc cgcccagatg acgacctcct cttcaccgtc ttctccaagg gccagaagcg
     2641 gaagatgaaa tctctggatg agtctgccct gtgcatcttc atcttgaagc agatcaatga
     2701 tcgcattaag gaccgcctgc agtcctgtta taggggtgag ggaacactag acctggcctg
     2761 gctcaaggtg aaggacatcc cttgcagcag tgcgctcctt actattgatg acaacttctg
     2821 tggcctggat atgaatgctc ctctgggagt ttccgaaatg gtgcgtggca ttccagtctt
     2881 cacagaagac agggaccgca tgacttctgt catcgcctat gtctacaaga accactcact
     2941 ggccttcgtg ggcaccaaaa gtggcaagct gaagaagatc cgggttgatg ggcccaaggg
     3001 aaatgccctc caatatgaga ctgtgcaggt ggtggactca ggaccagtcc tccgggacat
     3061 ggccttttcc aaggaccacg agcaactcta catcatgtca gaaaggcagc ttaccagagt
     3121 tcctgtggag tcctgtgggc agtaccggag ctgtggcgag tgtcttggct caggtgaccc
     3181 ccattgtggc tggtgtgtgc tccacaacac gtgcacccgg aaggaacggt gtgaacgctc
     3241 cagagaaccc cgaaggtttg cctcagagat gaagcagtgt gtccggctga cggtccatcc
     3301 caacaacatc tctgtctctc agtacaacgt gctgctggtc ctagagacat acaatgtccc
     3361 ggagctgtca gcaggtgtca actgtacctt tgaagatctg tcagagatgg atgggctggt
     3421 aataggcaat cagatccagt gctactcccc tgcagccaag gaagtgcccc ggatcatcac
     3481 agaaaatggg gaccatcatg ttgtccagct gcaactcaag tcaaaggaga ctgggatgac
     3541 cttcgccagc accagctttg tcttctacaa ctgcagtgtc cacaattcat gcctgtcctg
     3601 tgtggagagc ccgtaccgtt gccactggtg taaataccgg catgtttgta ctcacgaccc
     3661 caacacatgc tccttccagg aaggccgagt gaagctacct gaggactgcc cccagctgct
     3721 gcgagtggac aagatcctcg ttccagtaga ggtgatcaag cccatcactc tgaaggctaa
     3781 gaacctccca cagcctcagt cgggacagag aggctacgag tgcatcttga acatccaggg
     3841 catcgagcag agggtacctg ctctgcgctt caacagctcc agtgtgcagt gtcagaacac
     3901 ctcctattcc tatgaaggaa tggagatcaa caacctgcca gtggagttga cagtcgtgtg
     3961 gaatggacac ttcaacattg acaacccagc tcagaataaa gtttacctct acaagtgtgg
     4021 agccatgcgc gagagctgtg ggctgtgcct caaggccgac ccggatttcg agtgtggctg
     4081 gtgtcagagc ccaggccagt gtaccctgcg ccagcactgc cctgcccatg agagccggtg
     4141 gttggaactg tcgggggcca acagcaagtg caccaaccct cgtatcacag agatcatccc
     4201 agtgacgggc cctcgagaag ggggcaccaa ggtcaccatc cgaggggaga acttgggcct
     4261 ggaattccgt gacattgcat cacacgtgaa agtggctggt gtggaatgca gccccttggt
     4321 ggatggctac atcccagctg aacagatcgt gtgtgagatg ggtgaggcca agcctagcca
     4381 gcatgcaggc tttgtggaga tctgtgtggc tgtgtgtcgg cctgagttca tggcccgctc
     4441 ttcgcagctc tattacttca tgactctgac cctcgcagat ctgaagccca accgaggacc
     4501 catgtctggg gggacacagg ttaccatcac aggtaccaac ttgaatgcag gcagcaatgt
     4561 tgtggtgatg ttcgggagcc agccctgcct cttccacagg cgctctccat cctacattat
     4621 ctgtaacact acatcctcag aggaggtgct ggacatgaag gtgactgtgc aggtggacag
     4681 ggccaggatc cgccaggacc tggtctttca gtacgtggag gaccccacca ttgtgcggat
     4741 tgaaccagaa tggagcattg tcagtgggaa cacacctatt gctgtctggg gaactcacct
     4801 ggacctcata cagaacccac agatccgtgc caagcatgga ggaaaagaac acatcaacat
     4861 ctgtgaggtc ctaaatgcta cagagatgac ctgccaggct ccagcccttg ccctgggtcc
     4921 cgaccaccag tcagatctca ctgagaggcc tgaagaattt ggtttcatcc tggacaatgt
     4981 ccagtctctg ctaattctca acaagaccaa cttcacctat tatcctaacc ccgtgtttga
     5041 ggctttcagt ccctcgggaa tcctggagct caagccgggc acccccatta tcctaaaggg
     5101 caagaacttg atcccgcctg tggccggagg caatgtgaag ctgaactaca ccgtgctggt
     5161 tggggagaaa ccatgcaccg tgacagtatc agatgtgcag ctgctctgcg agtctcccaa
     5221 cctcatcggc aggcacaaag tgatggcacg ggtgggtggc atggagtatt cccctgggat
     5281 ggtatacatc gccccagaca gcccgctcag cctgggcgcg cctgacaaaa ctcacacatg
     5341 cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa
     5401 acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt
     5461 gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa
     5521 tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct
     5581 caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa
     5641 agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc
     5701 acaggtgtat accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac
     5761 ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca
     5821 gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct
     5881 ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc
     5941 cgtgatgcat gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg
     6001 taaaggcggc catcatcacc atcaccatta atctagagat cttattaaag cagaacttgt
     6061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6181 tctggtcgac tctagctaga ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6361 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6961 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7021 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7081 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7141 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7201 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7261 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7321 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7381 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7441 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7501 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7561 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7621 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7681 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7741 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7801 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7861 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7921 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7981 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8041 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8101 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     8161 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     8221 gacggtgaaa acctctgaca catgcagctc ccggaggaat gtgtgtcagt tagggtgtgg
     8281 aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8341 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8401 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8521 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8581 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cgtcccggag acggtcacag
     8641 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8701 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8761 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     8821 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8881 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8941 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     9001 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     9061 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9121 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9181 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9241 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     9301 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9361 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9421 gccagtgaat t
//
