LOCUS       sequence-139274-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinB3 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb3-Fc-His
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6032..6049
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6075..6196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6112..6131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6166..6185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6234..6251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6405..6993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6485..6504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7164..7955,7956..8024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7787..7806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8025..8129)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8197..8215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8253..8275)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8277..8297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8282..8612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8463..8598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8525..8544
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8749..8768
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8835..9290)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8972..8993)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9184..9203
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9416..9438
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9430..9447
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9431..9447
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgctgactga
     1621 cttcctccag gctcctgtga tggctccctg gtctcccttc agcctccacc tgctgctgct
     1681 gttcctgccg ctgctgcctt tgaccagggt ccatcgcttc tctgtaccaa ataccagctt
     1741 caaccacctg gtgctggcac cagatcaggg caaactctac gtgggtgcag tgaatcacct
     1801 ctttcagctc agccctgagc tgaagatgga gtctgtggct gtcactggcc ctgtcattga
     1861 cagtcctgat tgtgtgcctt tccgtgacct ggctgagtgc ccacaggccc agctcactga
     1921 caatgccaac cagctcttgt tggtgagcag caggacacag gaactggtgg cttgtgggca
     1981 agtgaagcag ggtgtatgtg agaagcggcg ccttggggat gtgactcaag tgttgtacca
     2041 ggctgaggac cctggtgatg ggcagtttgt ggctgccaat accctgggag tgaccacagt
     2101 aggcctagtg gtgcccttgc caggccgaga cctcctgcta gtggccagag gcctggcagg
     2161 caagctgtca gcaggagtgc cacccctaac tgtgcgccag ctggctgggc cacagccttt
     2221 ctccagtgaa ggactgggaa gacttgtggt cggtgacttt tcagactaca acaatagcta
     2281 tgtaggagct ttttctgatg ctcactctgc ctactttgtg ttccgtcgcc gtggggctcg
     2341 ggcccagact gagtatcgct cctatgtagc tcgtgtctgc ctcagagatg tcaaccttta
     2401 ttcctatgtg gaaatgccac ttacctgcca tggccagggc cttatccagg ctgccttcct
     2461 tactccagac actttgctag gtgcattttc agcaggcaca agtcaggcac aggcggccct
     2521 gtgtgccttt cccttagctg acctggacag gagtatggaa caagccaggc gactttgcta
     2581 tactactggt ggtcagggtc ctagcggcat ggaagaagcc actgtggagt atggtgtcac
     2641 atcccgttgc gtcactttgc ctcctgactc ccccgagtca tacccctgtg gtgatgaaca
     2701 cacccccagc cccatcgcag gccgccaacc cctggaggcc cagcctctgc tgcaactggg
     2761 acagtccatc agtgcagtgg ctgctctcca gacagatggg cacacgatag ccttcctggg
     2821 agacacccaa ggccagctac ataaggtctt tctaaacagc tcccatggcc aggtatacca
     2881 ttcccagcaa gtggggcccc caggctcagc catcagtcca gatctgctgg tagacagcaa
     2941 tggagaccac ctctatgtct tgactgccca gcaggtagac cggatacttg tggcagcctg
     3001 tcctcagttt cctaactgca ctacctgtct ccaggccagg gacccactat gtggttggtg
     3061 catccttcag ggaaggtgta ctcggagagg cgagtgtgga cgggcagccc agccaaacca
     3121 ctggctgtgg agctacgaag acaaccactg cccttacatc cagagcctgc taccagccca
     3181 acacccccgt caggaacagg gtcagatcat tctgtctgtt cctcggctgc ccaccctggc
     3241 catggatgaa tacttccact gtgcttttgg gggctacaac agtctagctc aggtggaaga
     3301 accccatgtg gtctgtacca cccctcctca agatcagatg ccacctaatc ctccaggctc
     3361 agatcatgtc accttgcccc tggccctaat gtttgaggat gtggtattga ccgccaccac
     3421 attctccttc tatgactgca gtgctgttca ggccttggaa gtggctgccc cgtgccgcgc
     3481 ttgtgtgagc agcctgtggc ggtgccactg gtgcccccag agtagccact gtatatatgg
     3541 agagcactgc ccagagggtg aaaaggctgt ctacagtgcc caggaggtgg acatcctggt
     3601 tcgtggccca gaggcttgcc cccaggttga gggtctagca agtccccagt tggtgcctgt
     3661 gggttgggag agccatgtga ccctgcacat tcagaacctt cactatttcc aaggtctgcc
     3721 tgccttgtac cactgctggc tagagctgcc tggaaaactg caaaagctac ctgcctcctt
     3781 agaggagaca tccagggact ctggcctcat tcactgccag gcccagcagt tctacccctc
     3841 tatgtctcag tgggagctcc cagtgcccat ctatgtaacc aggggtgaga tccagcggct
     3901 ggacaatgct ggtgatcttc atgtgactct atatgactgt gccatgggtc accctgactg
     3961 cagccactgc caggcggcca atgggagcct gagctgtttg tggtgtggtg atggccagcc
     4021 tgcctgccgc tatgggcctt tgtgcccacc aggtgctgtg gaacagctat gccctatacc
     4081 cagcattgat gtgattgaac ccctgactgg tcccccagaa ggtggcttgg ccatcaccat
     4141 cctgggttcc aacctgggcc aggcctttaa tgatgtgcga aatgctgtaa ctgtggctgg
     4201 ccagccctgc aaccctgacc cttctctcta ccgcatctct gctcggattg tatgtgtgac
     4261 atctcctgct cccaatggca ctgctgggcc agtccaagtg gccattaaga gtcgtcctcc
     4321 tggcatctca acccagaact tcacctacca ggaccctgtc ctgctgagcc tgaatcctca
     4381 gtggggcccc caggcagggg gcacccagct caccatccat gggcagtatc tccagacagg
     4441 aggcaacatc agtgtctttg tgggtgacca accttgccct atccaggagc ctgtgtgtcc
     4501 tgaagccatc atatgccaca ccatgcccca gactgagcca ggagaagcag tggtcctcat
     4561 agtctttggg catgttgaac gcaaactgct caccacccct tttcgataca cagccaatcc
     4621 ccaactggtg gaagcagagc ctagtgtcag cttccgggga ggtggacgag tgattcgagt
     4681 caggggtaca ggcttagacg ttgtgtggca gcccttgctg tctgtgtggc tggaggatga
     4741 gcccaaagta aaggccttgg gggttcaggc ccaggatgcg aacccaagga ggagctgtgg
     4801 tgcccctgct gcagatcctc aggcctgtat ccatcttgag agtggcttac tacagtgttc
     4861 cacgctctgc tctgtcaact catccagcct cctcctgtgc cacagtccag cagtgccaga
     4921 tggggccctc ccaaagcgag tcttctttgc cctagacaac atgcaagtag actttgccag
     4981 tgctagtggg ggccagggat tcctatacca gcctaaccct cgcctggctc cacttagtca
     5041 tgaggggatt actcacccct accacctcaa gccaggccat gtcctagatg tggagggcga
     5101 gggcctcaat ctggggatca gcaaggagga ggtgcaggtg catattggag atggagagtg
     5161 cctggtgaag acactgacgc ttactcactt gtactgtgag ccaccacccc aagcccctca
     5221 acccaccaat ggttcaggca ccctgccgca gtttgtggtg cagatgggca acctgcgcct
     5281 ggctctaggc cctgtccagt acgaagcaga gtccatgatg tccacctttc ctgtgggcgc
     5341 gcctgacaaa actcacacat gcccaccgtg cccagcacct gaactcctgg ggggaccgtc
     5401 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
     5461 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
     5521 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac
     5581 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta
     5641 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
     5701 caaagggcag ccccgagaac cacaggtgta taccctgccc ccatcccggg atgagctgac
     5761 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
     5821 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
     5881 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca
     5941 ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa
     6001 gagcctctcc ctgtctccgg gtaaaggcgg ccatcatcac catcaccatt aatctagaga
     6061 tcttattaaa gcagaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6181 tcatcaatgt atcttatcat gtctggtcga ctctagctag actcttccgc ttcctcgctc
     6241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8161 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8221 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggaggaa
     8281 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa
     8341 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8401 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8521 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8581 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8641 tcgtcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     8701 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     8761 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     8821 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     8881 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     8941 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     9001 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     9061 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9121 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9181 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     9241 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     9301 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9361 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9421 tcacgacgtt gtaaaacgac ggccagtgaa tt
//
