LOCUS       sequence-139285-         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 3A2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-3A2
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(240..318)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
       61 tcttgcggga ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc
      121 taaatgtaga atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg
      181 ttttgaaagc gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag
      241 caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat
      301 gctgttttga atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag
      361 aaattcaaag aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa
      421 taaactactt tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat
      481 attttttgaa gagatattt
//
