LOCUS       sequence-139286-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 1E4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-1E4
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(296..374)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
       61 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      121 gcgggattac gaaatcatcc tgtggagctt agtaggttta gcaagatggc agcgcctaaa
      181 tgtagaatga taaaaggatt aagagattaa tttccctaaa aatgataaaa caagcgtttt
      241 gaaagcgctt gtttttttgg tttgcagtca gagtagaata gaagtatcaa aaaaagcacc
      301 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatgctg
      361 ttttgaatgg ttccaacaag attattttat aacttttata acaaataatc aaggagaaat
      421 tcaaagaaat ttatcagcca taaaacaata cttaatacta tagaatgata acaaaataaa
      481 ctacttttta aaagaatttt gtgttataat ctatttatta ttaagtattg ggtaatattt
      541 tttgaagaga tattttgaaa aagaaaaatt aaagcatatt aaactaattt cggaggtcat
      601 taaaactatt attgaaatca tcaaactcat tatggattta atttaaactt tttattttag
      661 gaggcaa
//
