LOCUS       sequence-139287-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant G6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-G6
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(256..334)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
       61 ccggtactgc cgggcctctt gcgggattac gaaatcatcc tgtggagctt agtaggttta
      121 gcaagatggc agcgcctaaa tgtagaatga taaaaggatt aagagattaa tttccctaaa
      181 aatgataaaa caagcgtttt gaaagcgctt gtttttttgg tttgcagtca gagtagaata
      241 gaagtatcaa aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
      301 tattttaact tgctatgctg ttttgaatgg ttccaacaag attattttat aacttttata
      361 acaaataatc aaggagaaat tcaaagaaat ttatcagcca taaaacaata cttaatacta
      421 tagaatgata acaaaataaa ctacttttta aaagaatttt gtgttataat ctatttatta
      481 ttaagtattg ggtaatattt tttgaagaga tattttgaaa aagaaaaatt aaagcatatt
      541 aaactaattt cggaggtcat taaaactatt attgaaatca tcaaactcat tatggattta
      601 atttaaactt tttattttag gaggcaaa
//
