LOCUS       sequence-139288-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pTARBAC2 with GalK selection marker and 
            lens-specific transgenesis marker Cerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..713,714..716,717..719))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(45..66)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     394..413
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     653..674
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
       61 accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag
      121 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      181 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg
      241 ttcttctgct tgtcggcggt gatatagacg ttgtcgctga tggcgttgta ctccagcttg
      301 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      361 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      421 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      481 ttcatgtggt cggggtagcg ggcgaagcac tgcacgcccc aggtcagggt ggtcacgagg
      541 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      601 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      661 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
      721 gtggcgaccg gtggatccac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg
      781 aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc
      841 tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc
      901 tgacctga
//
