LOCUS       sequence-139296-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Deoxyviolacein pathway (vioABEC) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE) and 4A6 (vioC) for orthogonal flux redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtttcaattc ggccagccgg ttgacattga cttgccgccg gcacgtttcc acgaatacct
       61 ccactcactt ggtccaaaag ttaatcgctt tggtaaaaaa gatgaaaatg gagaattcag
      121 tgtagcgtgt ggctacaaca tgcttggtaa taatcatttt tcatggcacg acgctaaagt
      181 tacggctttg caattgcgcc cgggtgattg tgttgttcaa gatccactca tagggtgttc
      241 tgttaattta tggggacact acaatgagta ccttagaacc tcattcaatc gtgctcgctg
      301 ggttgataat gacccgaccc gaagtgactc ggcactcatc tatgctggac aaattacatt
      361 aactgcaaat ggcagtgagg caagtgaaaa gcatgtgatg tctgctgaca ttgattgtac
      421 acatggagtg agatggataa atcctagctt tattaatagc ctcgacgaac atgcgtttgc
      481 tgaagatatt gcagcagccc gtttgtttca
//
