LOCUS       sequence-139297-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 5F5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-5F5-mCherry
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             116..138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cggccacgat gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa
       61 tacgactcac tatgtgggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggagata tacatatggt ttcaaaaggc gaagaagaca acatggcgat
      181 tatcaaggaa tttatgcgtt tcaaggtcca catggaaggc agcgtcaatg gtcacgaatt
      241 tgaaattgaa ggcgaaggtg aaggccgtcc gtatgaaggc acccagacgg caaaactgaa
      301 ggtcaccaaa ggcggtccgc tgccgtttgc ttgggatatt ctgtcaccgc aattcatgta
      361 tggttcgaaa gcgtacgtta agcatccggc cgatatcccg gactatctga aactgtcctt
      421 tccggaaggc ttcaaatggg aacgtgttat gaacttcgaa gatggcggtg tggttaccgt
      481 cacgcaggat agctctctgc aagacggtga atttatttat aaagtgaagc tgcgcggcac
      541 caatttcccg agcgatggtc cggttatgca gaaaaagacg atgggctggg aagcgagttc
      601 cgaacgtatg tacccggaag acggtgccct gaaaggcgaa atcaagcagc gcctgaaact
      661 gaaggatggc ggtcactatg acgcagaagt gaaaaccacg tacaaggcta aaaagccggt
      721 ccaactgccg ggtgcataca acgtgaacat c
//
