LOCUS       sequence-139299-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains a Cas9 Activator for yeast. The activator is 
            about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It
            is on a single copy CEN/ARS plasmid with Ura marker, pRS416..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Naranjo S, Smith JD, Artieri CG, Zhang M, Zhou Y, Palmer ME, Fraser 
            HB
  TITLE     Dissecting the Genetic Basis of a Complex cis-Regulatory Adaptation.
  JOURNAL   PLoS Genet. 2015 Dec 29;11(12):e1005751. doi: 
            10.1371/journal.pgen.1005751. eCollection 2015 Dec.
  PUBMED    26713447
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-12-29"; volume: "11(12):e1005751. doi"; pages: 
            " 10.1371/journal.pgen.1005751. eCollection 2015 Dec"
COMMENT     Sequence Label: pRS416-Gal4-dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..87
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             97..438
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    454..478
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttttcttct tgctcattag aaagaaagca tagcaatcta atctaagttt tctagaacta
       61 gtgatgccca agaagaagcg gaaggtctcg agcggcgcca attttaatca aagtgggaat
      121 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
      181 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
      241 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
      301 ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
      361 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
      421 ccaaacccaa aaaaagaggg tgggtcgaat caaacaagtt tgtacaaaaa agcaggctct
      481 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc
      541 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc
      601 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggg
//
