LOCUS       sequence-139302-       10976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Combined sgRNA/Cas9 vector with KanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10976)
  TITLE     sgRNA/Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMZ376
FEATURES             Location/Qualifiers
     source          1..10976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     175..197
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     189..206
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     190..206
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(256..307)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(426..782)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(819..835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(819..835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(832..854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    843..859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(867..873,874..891,892..897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    912..933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1050..1067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1221..1809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1301..1320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1980..2771,2772..2840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2603..2622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2841..2945)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2974..3715
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             3736..7839
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3736..7839
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3736..7839
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7852..7872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7905..8152
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7921..7939)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     8199..8217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8255..8277)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8377..8396
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8765..9546
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     gene            9620..10976
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        9620..9963
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             9964..10773
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10031..10050)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      10779..10976
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
       61 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
      121 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      181 cacgacgttg taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gactctagag
      241 gatccccacg gtacccctgt ttcgtcctca cggactcatc agaccggaaa gcacatccgg
      301 tgacagggca ccaccgactc ggtgccactc acttttttca agttgataac ggactagcct
      361 tattttaact tgctatttct agctctaaaa cagagagata acccacaaga attgagttaa
      421 gcccattctt cggtacaggt tatgtttttt ggcaacagca actattcaaa tcaactgaaa
      481 gttttttaga attcaacaac ttccaatact caagtgtcca agctagcaaa ctgtttaata
      541 atcgtgaccc aacattcctt tcatgcgcac cctcagcgtc acataaatca acactttaca
      601 acacctattc aaagcttcta tatgccttcg tcatttaaat gtctaacatt tcaaaaaagt
      661 gtttgctaat ggtaaattct tgagttttct tggaagcaca tatataacga gtgtgactgt
      721 tctacgctcc ttgctgacta gtcatcagtc tacgcatgcc aactaccacc aacataagca
      781 aaaggtaccc ggggtaccga gctcgaattc gtaatcatgt catagctgtt tcctgtgtga
      841 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      901 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1021 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1201 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1981 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2041 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2101 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2161 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2221 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2281 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2341 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2401 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2461 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2521 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2581 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2641 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2701 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2761 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2821 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2881 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2941 ccgcgcacat ttccccgaaa agtgccacct gacgccctac aacaactaag aaaatggcta
     3001 tcatgcggaa gctggtgaga agaacagcat cgggacaagg gaaggaagaa caaagacaaa
     3061 gaaaacaaaa gaaagcaatt gaaaacaaaa caaaacaatt ttcattcctt ctcttatcat
     3121 tccttttctt ttcttttctc tcattcaacg cactccatcg tatccgtatt cctcttattt
     3181 tttctctttc tctatatcca tttctttctc tctaggtgtg tcctctctct ctcttcaatt
     3241 tctctactcc gcattccaac gcatccttcc cccaacctcc catttcctcc ttacggcccg
     3301 atagcgatcg tctttccctc gctatcactc gctaccggcc cctcctctgc accgtaacct
     3361 cctacgtatt taccatatca taaagttttt tccgacgctt atcgctgacc ccctgtcgcc
     3421 ctcctattgg cttccggatt atcttcttgt ccataaggtg atccatgctt cctgaagatt
     3481 cccgaaatgt gtccactttg gcggggaatc attccatcca cttctttctc tctcgctttc
     3541 ctcattcggc gctccccttc cgcgtctcat tggtcttccg ctccgttttt gctttgccga
     3601 tgttacttgg ggagaggtgc gataatcctt tcgcaaaaac tcggtttgac gcctcccatg
     3661 gtataaatag tgggtggtgg acaggtgcct tcgcttttct ttaagcaaga gaattgctgc
     3721 aggtcgactc tagagatgga caagaagtac tccattgggc tcgatatcgg cacaaacagc
     3781 gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg
     3841 ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc
     3901 ggggagacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga
     3961 aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac
     4021 tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc
     4081 cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata
     4141 tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat
     4201 ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac
     4261 ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt
     4321 ttcgaagaga acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg
     4381 ctgtccaaat cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac
     4441 ggcctgtttg gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac
     4501 ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc
     4561 gacaatctgc tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac
     4621 ctgtcagacg ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct
     4681 ccgctgagcg ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg
     4741 aaggcccttg tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct
     4801 aaaaatggct acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt
     4861 attaagccca tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga
     4921 gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac
     4981 ctgggcgaac tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat
     5041 aacagggaaa agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc
     5101 gcccggggaa attccagatt cgcgtggatg actcgcaaat cagaagagac catcactccc
     5161 tggaacttcg aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg
     5221 actaactttg ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac
     5281 gagtacttca cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga
     5341 aagccagcat tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg
     5401 aaccggaaag ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat tgaatgtttc
     5461 gactctgttg aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac
     5521 gatctcctga aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt
     5581 cttgaggaca ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc
     5641 ttgaaaactt acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga
     5701 tatacaggat gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga taagcagtct
     5761 ggaaagacaa tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag
     5821 ttgatccatg atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc
     5881 cagggggaca gtcttcacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag
     5941 ggaatactgc agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag
     6001 cccgagaata tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag
     6061 aacagtaggg aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc
     6121 cttaaggaac acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac
     6181 ctgcagaacg gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac
     6241 tacgacgtgg atcatatcgt gccccagtct tttctcaaag atgattctat tgataataaa
     6301 gtgttgacaa gatccgataa aaatagaggg aagagtgata acgtcccctc agaagaagtt
     6361 gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg
     6421 aagttcgata atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc
     6481 ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc
     6541 gattcacgca tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt
     6601 attactctga agtctaagct ggtctcagat ttcagaaagg actttcagtt ttataaggtg
     6661 agagagatca acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact
     6721 gcacttatca aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg
     6781 tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag
     6841 tacttctttt acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga
     6901 gagattcgga agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac
     6961 aagggtaggg atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt
     7021 aaaaagaccg aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac
     7081 agcgacaagc tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat
     7141 tctcctacag tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa
     7201 aaactcaaaa gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa
     7261 aaaaacccca tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc
     7321 attaagcttc ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct
     7381 agtgcgggcg agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc
     7441 ttgtatctgg ccagccacta tgaaaagctc aaagggtctc ccgaagataa tgagcagaag
     7501 cagctgttcg tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa
     7561 ttctccaaaa gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat
     7621 aagcacaggg ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg
     7681 accaacttgg gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg
     7741 tacacctcta caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc
     7801 tatgaaacaa gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag
     7861 aagaggaagg tgtgattttg gacctcgagt cattggacct cgagtcatgt aattagttat
     7921 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag
     7981 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
     8041 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
     8101 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcgatccccg
     8161 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8221 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     8281 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     8341 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     8401 gagtgcacca taatcgatgg ccaaccttcc aattcattaa atcgagtcct ccatcagatg
     8461 atttacaagg ttttgcatag aatccctaag taagagaagt tgttagtgat ttcttcaagt
     8521 ttactaaaat ttacatacaa atggatgatc tcttctccat tgtttccttt cttcttgcaa
     8581 acgcgtttta caaagagatg acatgctatt ttctgattat ttttttctat agttttctat
     8641 tttgtacgtc cttgggagtc cgaaatgtaa aatcggggta tggttagtag cggcagcgtt
     8701 cattatggta tataaaagat aaaaagtagg ggcaagcgag acagaatggg atacaagggc
     8761 atcgtctata attatagcta aaaattgtat tttaatttgt attttttgta attttatttc
     8821 tcattgtttt acttaaaatg aatgcgaatt agagaaaact tcaacgaaat gtcaaaataa
     8881 gctcagcaaa atatacaatt ttagggaaag cgatcagcaa cttattctcc gttgtagatt
     8941 ctttttcttc ataaattcca acaatgtaac tgccaattga tttttctcta gatgctaata
     9001 aattgaaact aatgaatata ataacacagg ttttgataaa ttgtaaactc ttactcaaaa
     9061 aaagaatgtt aaaactaatt taaccttttt ataaaggctc atggcgttaa agaggatagt
     9121 aaaaattcta caatttttta atttaatgtc acaattgtct tgcgaatata attctattaa
     9181 ttttcgctaa ctgctgtttt agtactatta ctttcaatat gccttatatg caactttaat
     9241 tcatgaagaa tgcaatattt ttgcaatctt tcttgtactg agcgcttttt atcatttagt
     9301 actcattatt taatttttca gtaaagggca ataaataaat ttgtagaaga tgcaatgtaa
     9361 tctcctctat ccttttgctc atatgtttat gagtatacct agtctagaaa ggcttgtatt
     9421 taaaatatta ttcaataaaa attcacaatt tttacgacat gtgctaccat tcacttaact
     9481 tcctgattat aaaattggtt cgtttatact aattacttaa gtacctttaa ctaaacaaaa
     9541 tgcctatata tatattaatt tacaatgagt gtcagataag tcactatgtc cgagtggtta
     9601 aggagttaga ctcgccatcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
     9661 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
     9721 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
     9781 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
     9841 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
     9901 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
     9961 accatgggta aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct
    10021 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
    10081 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
    10141 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
    10201 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
    10261 cccggcaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
    10321 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
    10381 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
    10441 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
    10501 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
    10561 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
    10621 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
    10681 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
    10741 cagtttcatt tgatgctcga tgagtttttc taatcagtac tgacaataaa aagattcttg
    10801 ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa
    10861 atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa
    10921 gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactg
//
