LOCUS       sequence-139308-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, E. coli Dihydroneopterin triphosphate 
            pyrophosphatase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Gabelli SB, Bianchet MA, Xu W, Dunn CA, Niu ZD, Amzel LM, Bessman MJ
  TITLE     Structure and function of the E. coli dihydroneopterin triphosphate 
            pyrophosphatase: a Nudix enzyme involved in folate biosynthesis.
  JOURNAL   Structure. 2007 Aug;15(8):1014-22.
  PUBMED    17698004
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2007-08"; volume: "15(8)"; pages: "1014-22"
COMMENT     Sequence Label: pET11_DHNTPase
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             489..511
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    526..550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(550..569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(551..569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(653..672)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg
       61 cctgccggtt gctccaggac ttagtgagcg ccgccgcagc aggcgcatca agccacttgt
      121 aagccagatg ttcagtgaaa acgatctgcc gctcgtgcgg aagcgcaaga cagaaccatg
      181 attccgtatt acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa
      241 actctaccgt gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga
      301 cctcttcctt tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc
      361 cggttaccga ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg
      421 tatcttgtgc gtagatgacc actaagatcg aaacgggacg cttatacact ttatccttca
      481 tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      541 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      601 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      661 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      721 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      781 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactgg
//
