LOCUS       sequence-139317-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 586, Emission: 632.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: tdKatushka2-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..749
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             45..749
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg ggtgaggata
       61 gcgtgctgat caccgagaac atgcacatga aactgtacat ggagggcacc gtgaacgacc
      121 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
      181 agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
      241 tcatgtacgg cagcaaaacc tttatcaacc acacccaggg catccccgac ttctttaagc
      301 agtccttccc tgagggcttc acatgggaga ggatcaccac atacgaagac gggggcgtgc
      361 tgaccgctac ccaggacacc agcctccaga acggctgcct catctacaac gtcaagatca
      421 acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      481 ccagcaccga gatgctgtac cccgctgaca gcggcctgag aggccatgcc cagatggccc
      541 tgaagctcgt gggcgggggc tacctgcact gctccctcaa gaccacatac agatccaaga
      601 aacccgctaa gaacctcaag atgcccggct tctacttcgt ggacaggaga ctggaaagaa
      661 tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaggtact
      721 gcgacctgcc tagcaaactg gggcacagcg ggcatggcac cggcagcacc ggcagcggca
      781 gctccggtac cgcctcctcc gaggacaaca acatggccat ggtgggtgag gatagcgtgc
      841 tgatcaccga gaacatgcac atgaaactgt aca
//
