LOCUS       sequence-139328-        9335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinB2 protein (ie, 
            long), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb2(L)-Fc-His
FEATURES             Location/Qualifiers
     source          1..9335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3097
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5915..5932
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5958..6079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5995..6014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6049..6068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6117..6134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6288..6876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6368..6387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7047..7838,7839..7907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7670..7689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7908..8012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8080..8098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8136..8158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8160..8180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8165..8495
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8346..8481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8632..8651
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8718..9173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8855..8876)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9067..9086
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9299..9321
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9313..9330
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9314..9330
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggctctgcc
     1621 actctgggcc ctgacctttc taggtctcac gggcctaggg ttgagcctgc ggtcccgaaa
     1681 gccggagagt ttccgcagtg agacagagct gaaccacctg gctgtggatg aggtcacagg
     1741 tgtggtgtat gtcggggcag tgaacgcgct ctaccagctg agtgccgacc tgcacgtcca
     1801 gcagcacgtg gtcacaggcc ccttcatgga taacaagaag tgcacaccac ccattgaggc
     1861 cagccagtgc cacgaagcgg tgctcactga caacttcaac cagctgctgc tgctcgaccc
     1921 accggggaaa cgcctggtgg agtgtggcag cctcttcaag ggcatctgtg ccctgcgcgc
     1981 catgagcaac atctcggtgc gcctcttcta cgaggatggc agcggcgaga agtccttcgt
     2041 ggccagcaat gacgagagag tggccaccgt ggggctggtg acctccacgc gccccgacgg
     2101 cgagcgcgtg ctgtttgtgg gcaaaggcaa tgggccgcac gacaatggta tcatcgtgag
     2161 cacccgcctg ctggacaggg ctgagggccg ggaggccttt gaggcctact ctgaccacac
     2221 caccttcaag gccagctacc tgtccacgaa tactcagcag tttgtcgcag cctttgagga
     2281 tgatttctat gtcttcttcg tctttaacca tcaagacaag catcctgcta agaaccgcac
     2341 gctgctggcg cgcatgtgca aagacgaccc ctcctactat tcctatgtag agatggacct
     2401 gcagtgtcag gatcccagtg acccccaaga ctctgccttt ggcacctgcc tagccgcttc
     2461 tgtagccaca tcgggtgctg gcagagcact ttatgctgtc ttcagcagag atggccggag
     2521 cactggggga cctggcgcgg gcctctgtgt gttcccgcta gataaagtcc gtgagaagat
     2581 agaagccaac cgtaacgcct gctacacagg tgcccgagag gcaggccgca ctatcttcta
     2641 caagcccttc cacggagaaa tccagtgtgg cggccatctg ataggtgcca gcgagagctt
     2701 cccatgcggc tcggagcatc tgccctatcc actgggcagt cgtgatggac tcgtcgccac
     2761 agccgtgctg caccgcgggg gcctgaatct gacagcagtg acagtaactg ccgagaatga
     2821 ccacactgtt gcgttcctgg gcacctcaga tggccggatc cttaaggtgt accttgctcc
     2881 agacggcact tctgcagagt atggttctat cccagtagac atcaataaga aaatcaagca
     2941 ggacctggca ctgtctggaa acctgtccag tctgtatgct atgacccagg acaaggtgtt
     3001 ccggctccca gtacaagaat gcctgagcta tgtaacctgc gctcagtgtc gtgactccca
     3061 agatccctac tgtggctggt gtgtcatcga gggacgatgc accaggaagt ccgagtgctc
     3121 acgggccgag gaaaccggac actggctgtg gagccgggag aagtcctgtg tggccatcac
     3181 tgatgccttt ccacagaaca tgagccggcg ggcccaagga gaggtacgcc tgtctgtcag
     3241 ccccctgccc accctgactg aggacgatga gttactgtgc ctctttggtg actcaccacc
     3301 ccaccctgcc cgggtagagg acgataccgt catctgtaat tccccaagca gcatccctag
     3361 tacaccgcca ggccaagacc atgttgacgt gagcatccag ctcctcttaa aaagcggcag
     3421 tgtcttcctc acctcccacc agtatccctt ctatgactgc cgtgaggcca tgagcctggt
     3481 ggagaacctg ccgtgcatct cttgtgctag caaccgctgg acttgccaat gggacctgca
     3541 gtactacgag tgtcgggagg cttcgcccaa cccagaggag ggaatcatac gtgcccacat
     3601 ggaggacaac tgcccccagt tcctggcccc tgaccctttg gtcatcccca tgaaccatga
     3661 gacagaggtg accttccagg gcaagaactt ggagactgtg aaggtttctt ccctgtatgt
     3721 gggcagtgag ctgctgaatt ttgaagagac tgtgaccatg catgaatcag acaccttctc
     3781 ttttaggacc ccaaagctat cccatgatgg taatgagaca ctgcctcttc acctgtatgt
     3841 taagtccttt ggcaagaaca ttgacagcaa gctacaagtg actctctaca actgctcctt
     3901 tggccgcagt gactgtagcc tgtgtctggc tgccgatcct gcctacaggt gtgtgtggtg
     3961 ccgtgggcag aaccggtgcg tgtacgaagc tctgtgcagc aatgtcactt ctgagtgccc
     4021 accaccagtt atcactagga tccagcctga gacgggcccg ctgggtgggg gcatcctggt
     4081 cactatccat gggtccaatc tgggtgtcaa agcagatgac gtcaagaaga taactgtggc
     4141 tggccagaac tgtgcctttg aaccaagagg gtactccgta tccacccgga ttgtgtgtgc
     4201 aattgaggct tcggagatgc ccttcacagg aggcattgag gtggatgtta atggaaaact
     4261 cggccattca ccgccacacg tccagttcac ttatcaacaa ccccagcctc tcagtgtgga
     4321 gccacgacag gggccacagg caggtggcac cacattgacc atcaatggca ctcacctgga
     4381 cacaggctcc aaggaggatg tgcgggtgac actcaatgac gtcccttgtg aagtgacaaa
     4441 gtttggagca cagctacagt gtgtcacagg tcaacagttg gctccaggcc aggtgacact
     4501 agaaatctac tatgggggct ccagagtgcc cagccccggc atctctttca cctactgcga
     4561 gaaccccatg atacgagcct ttgagccatt gagaagcttt gtcagtggtg gccggagcat
     4621 caacgttact ggccagggct tcagcctcat ccagaagttt gccatggttg tcatcgctga
     4681 gcccttgcgg tcctggaggc ggcggcggcg ggaggctgga gccctggagc gtgtgacggt
     4741 cgagggcatg gagtacgtgt tctacaacga caccaaggtc gtcttcttgt ctcctgctgt
     4801 ccccgaagag cccgaggctt acaacctcac cgtgctgata cggatggacg gtcactgtgc
     4861 cccgctcagg accgaagccg gagtctttga gtatgtggca gaccccacct ttgagaactt
     4921 cacgggtggc gtcaaaaagc aggtcaacaa gctcatccac gcccggggaa ccaatctgaa
     4981 caaagccatg actctggagg aggcagaggc atttgtgggt gctgagcgtt gcattatgaa
     5041 gacgctgacc gagacggacc tgtactgcga gcccccagag gtgcagcctc cacccaagcg
     5101 gcggcagaag cgagacacgg cacacaacct acctgagttc attgtgaagt ttggctcccg
     5161 agagtgggtg ctaggccgag tggagtacga cacgcgtgcg agtgacgtgc cgctcagtgg
     5221 cgcgcctgac aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc
     5281 gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga
     5341 ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta
     5401 cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag
     5461 cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga
     5521 gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa
     5581 agccaaaggg cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct
     5641 gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc
     5701 cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct
     5761 ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca
     5821 gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca
     5881 gaagagcctc tccctgtctc cgggtaaagg cggccatcat caccatcacc attaatctag
     5941 agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6061 aactcatcaa tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg
     6121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7021 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7081 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7141 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7201 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7261 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7321 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7381 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7441 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7501 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7561 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7621 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7681 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7741 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7801 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7861 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7921 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7981 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8041 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8101 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8161 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc
     8221 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     8281 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     8341 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8401 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     8461 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     8521 ttttcgtccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8581 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     8641 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8701 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8761 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8821 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8881 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8941 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9001 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9061 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9121 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     9181 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     9241 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     9301 cagtcacgac gttgtaaaac gacggccagt gaatt
//
