LOCUS       sequence-139332-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 1D4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-1D4-mCherry
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtacctta tttgtacagt tcatccatac cgccggtaga gtggcggcct tctgcacgtt
      121 cgtactgttc aacgatcgta tagtcttcgt tatggctggt gatatccagc ttgatgttca
      181 cgttgtatgc acccggcagt tggaccggct ttttagcctt gtacgtggtt ttcacttctg
      241 cgtcatagtg accgccatcc ttcagtttca ggcgctgctt gatttcgcct ttcagggcac
      301 cgtcttccgg gtacatacgt tcggaactcg cttcccagcc catcgtcttt ttctgcataa
      361 ccggaccatc gctcgggaaa ttggtgccgc gcagcttcac tttataaata aattcaccgt
      421 cttgcagaga gctatcctgc gtgacggtaa ccacaccgcc atcttcgaag ttcataacac
      481 gttcccattt gaagccttcc ggaaaggaca gtttcagata gtccgggata tcggccggat
      541 gcttaacgta cgctttcgaa ccatacatga attgcggtga cagaatatcc caagcaaacg
      601 gcagcggacc gcctttggtg accttcagtt ttgccgtctg ggtgccttca tacggacggc
      661 cttcaccttc gccttcaatt tcaaattcgt gaccattgac gctgccttcc atgtggacct
      721 tgaaacgcat aaattccttg ataatcgcca tgttgtcttc ttcgcctttt gaa
//
