LOCUS       sequence-139345-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector expression sgCentromere-20xPBSc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1454_pX-sgCentromere-25xPBSc
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..138
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
       61 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      121 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      181 gaaaggacga aacaccgttg aggccttcgt tggaaacgtt taagagctat gctggaaaca
      241 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      301 tgccaattgg gtctccagat ttgatgtagc cttgatgtag ccttgatgta gccttgatgt
      361 agccttgatg taagatttga tgtagccttg atgtagcctt gatgtagcct tgatgtagcc
      421 ttgatgtaag atttgatgta gccttgatgt agccttgatg tagccttgat gtagccttga
      481 tgtaagattt gatgtagcct tgatgtagcc ttgatgtagc cttgatgtag ccttgatgta
      541 agatcttttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgtagcgc
      601 gtgcgccaat tctgcagaca aatggcggcg cgccgcggcc gcaggaaccc ctagtgatgg
      661 agttggccac tccctctctg cgcgc
//
