LOCUS       sequence-139347-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Deoxyviolacein pathway (vioABEC) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE) and 4A6 (vioC) for orthogonal flux redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..167
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(206..224)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      220..267
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    320..344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             359..381
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tcacgggtga tccgaacgtt cacgcctctg taagagactt tcctaatttt aatacgctgc
       61 cgatacctaa cacgattttt gatgtaacaa gtctctctca taggaattga ctcgagtctg
      121 gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct actagtcgca
      181 gcttaattaa cctaaactgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      241 tctaaacggg tcttgagggg ttttttgcta ggatcgagat cgatctcgat cccgcgaaat
      301 taatacgact cactacccgg gaattgtgag cggataacaa ttcccctcta gaaataattt
      361 tgtttaactt taagaaggag atatacatat gagtaaaata attattgttg gtggtgggtt
      421 ggcaggcagc ctgacggcaa tctatctagc gaaacaaggg cacgaagttc acattataga
      481 aaaacgtgga aatcctttag aaagtcaggc ggattatatt gatcaagtta gcgcgcgtgc
      541 gattggtgtc agtatgacag tacgaggaat tgaagccgtt ttagcagccg gtattccctt
      601 ggacgaattg caggcgtgcg gcgttgaagt ttcaggtatg gcgtttttta tcaaaggtca
      661 acctaaaata cgggatttac caccgttaga tacgttaaaa ccgttgtcgt taagccgaag
      721 catgtt
//
