LOCUS       sequence-139351-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgat tagtattgaa acgcaaaacc cggatgttag caacctggac
      181 acctcgcact ctattccgaa aatggcaaac cgtattgatg accatgtgtt tcgttctaaa
      241 ctgccggaaa ttccgatcag taaccatctg ccgctgcaca cgtattgctt cgaaaattac
      301 tcgcagtttg cagaccgtcc gtgtctgatt gttggctcga cgaacaaaac ctatagcttc
      361 gctgaaaccc atctgatctc tcgcaaagtg ggcgcaggtt ttgctcacct gggtctgaaa
      421 cagggcgatg tggttatgat tctgctgcaa aattgcgcgg aatttgcctt cagctttctg
      481 ggtgcgtcta tggttggcgc cgtcaccacg accgcaaacc cgttctacac gtccgcggaa
      541 atcttcaaac agctgaacgc atcaaaagct aaaatcgtcg tgacccaggc gcaatatgtg
      601 gataaactgc gcgactaccc ggatggtcaa gttgccaaaa ttggcgaagg tttcacggtc
      661 attaccatcg atgacccgcc ggaaaactgt atgcatttta gtgttgtctc cgaagcgaac
      721 gaaagcgaac tgccggaagt ctcaattaat tcggatgacc cggtggccct gccgtttagc
      781 tctggtacga ccggcctgcc gaaaggcgtg gttctgacgc acaaatcact gatcac
//
