LOCUS       sequence-139360-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with 
            Arg232>His mutant (YfdE-R232H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Mullins EA, Sullivan KL, Kappock TJ
  TITLE     Function and X-ray crystal structure of Escherichia coli YfdE.
  JOURNAL   PLoS One. 2013 Jul 23;8(7):e67901. doi: 
            10.1371/journal.pone.0067901. Print 2013.
  PUBMED    23935849
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-23"; volume: "8(7):e67901. doi"; pages: " 
            10.1371/journal.pone.0067901. Print 2013"
COMMENT     Sequence Label: pJK560
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttatgat
       61 gagaactcct ggcgaatttg ttccccatgc tggtcgagcg ttgccgctcc cggcataaca
      121 tgcgggtccg cgcagccgct gatttttatc ggattacccg gcatcattat tcccccggct
      181 tcaatcaaca tatttctcgc ctgagtttgt ggcaatttaa tggcctcagc cacacttaat
      241 aacggcgcga cgggtacacc aacttcatgt attctggcta accaaacttc agctgcctgc
      301 gtttttaacg tccgctcaat atattgttta agaatagcct ggttttgtac gcgtaaaata
      361 ttgctgctaa atcggggatc attaaccagt tccgtaagct ccagtgcctg gcataacgca
      421 gaaaaaagct tgtcattacc acaacaaatc gtaatcggct tatcctgagt attgaaaaca
      481 tcaaaaggtg ccatgtaggg atggtgattt cccagacgtt gtggtgactt cccagttgcg
      541 atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat cgctatatcg
      601 acatgcgccc ctctctggct cttttcgcga ccataaagtg cactcactat tccgctgaat
      661 aaatagacac cgccgcatag atccgcaaga gatgtaccaa cgcgcactgg cggagcatca
      721 ggatatcctg tttccatcat tatcccgctc attgcctgaa tgatggtatc gtaggcagga
      781 gcatctttta gcggaccggt atgtccgaaa cctgacgatg aagcatatat gaggcgcggg
      841 ttgatttctt gaagcgtttc ccatgaaaac cccagttttt ccattgtacc tgggcgaaaa
      901 ttctcagcta atacatcagc ttgtttgagc atatttataa atatactttt atcgtgatca
      961 ttctttaaat caagaaccac actctctttg ccatgattaa taaaactgta atagagtgac
     1021 tgtccatcca catagggacc aaatgtgcgg gtatcatcac catgacccgg cggctcaact
     1081 ttaattaccc ttgcgcccat at
//
