LOCUS       sequence-139361-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             616..638
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    653..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(677..696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(678..696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcatttggcc cacggaaagt gaccgcggtg atttctgaac aaacaacaag gactcgagcg
       61 cccttgttgt tttcagccaa gtccttggct aaccgaagaa ccgtgccacc agcaaagcaa
      121 ccttgttggt acatcataag cctcttgacc gatggacgaa gcccaagtag cttagtgagt
      181 tgatagtcac acccaggcat gtccacacca ctggttgtgc aaaagaccaa atgggtaatt
      241 ttggacttgg gctggcccca ttccttgatg gccttttgag ctgcctcttt gccaagtttg
      301 ggcacttcaa ccaccactat gtcttgccta gcatcaagag aaggtgccat gtattcacac
      361 atactaggat tctctttcaa gatttcctct gttaagtgca tgtacctttt cttaatcatt
      421 gatttttcac acatgcgctt aaatttctcc ttaagatcag tcttgtgctc actgttagtg
      481 atacgaaaat aaaaatcagg gtaagtgctt tgatcaacac agtttgaagg tgtggctgtt
      541 ccaatggcca tgacagtggc tggaccttca gcacgttgtg ccttacgata ctcctcgact
      601 gtcaccatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      661 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct
      721 cgatcctagc
//
