LOCUS       sequence-139362-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the GPPS gene plus the lower MVA pathway from 
            Streptococcus pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  TITLE     Expression of the lower MVA pathway in Synechocystis PCC6803
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GlgA1-Ptrc-GPPS-FNI-MK-PMD-PMK-SmR-psbA2
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(546..551,552..568,569..575)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 catccccaac aaccagccat cagccttatt ctgttgctac gtcaatgcca ccaatcaaat
       61 ccaagtggcc cgcattacca atgtccctaa ttggtatttt gaaagagttg tgttccctgg
      121 tcaacggtta gtatttgagg cagtgcccag cgctcagtta gaaattcata ctggcatgat
      181 ggccagctcg attatttcgg acaccattcc ctgcgaacaa ctgagtattg atcccgacgg
      241 attagcagcg ggcggtttca tctctccaga aaaagaacac gagtccgagg atatgacttc
      301 ccaatcctta gtggcttagc aatgaattaa tgaattggaa tacttaggcc atgccaccgg
      361 ccggcaatgg atagtccacg gacaaagcac taagaaaaag gtatagggat ggaaagcaga
      421 aactgttaat tactctctcc gatgggtaac caccaccgtc atataattga gcggaaagta
      481 tggcaaccag gccctgaact caattagtgg aataacgcgg tcctgcagga ttctgaaatg
      541 agctgttgac aattaatcat ccggctcgta taatgtgtgg aaattgtgag cggataacaa
      601 ttaggaggtt aattaacaat gacgcgcagc agtaaggcct tggtccaact agctgatcta
      661 tccgaacaag taaaaaacgt ggtggaattt gattttgaca agtatatgca ctccaaggcc
      721 attgcggtta atgaggcctt agataaagtt attcc
//
