LOCUS       sequence-139363-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes, with N-terminal His6 tag (H6AaOrfX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK523
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             318..335
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(388..406)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      402..449
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      486..914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(573..592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     783..804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgctgcgtg atcgcgacct gctgctggcg
      121 caattgcatg aactgcgtag cgaacaccgc gatctggaca ccgtgatcaa ccgtatggcc
      181 catgaaacca cgtttatcga tcagctgtat ctgcaacgtc tgaaaaaacg caaactgctg
      241 cttaaggatc aaattacgaa agtggaaagt ctactgattc cggatgacat cgcgtgaagc
      301 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      361 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      421 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      481 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      901 atttaacgcg aattttaaca aaatattaa
//
