LOCUS       sequence-139364-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes, with N-terminal His6 tag (H6AaOrfX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK523
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(278..295)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(305..322)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             341..363
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    378..402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(402..421)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(403..421)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(505..524)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        730..807
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(827..846)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttca cgcgatgtca tccggaatca gtagactttc cactttcgta atttgatcct
      121 taagcagcag tttgcgtttt ttcagacgtt gcagatacag ctgatcgata aacgtggttt
      181 catgggccat acggttgatc acggtgtcca gatcgcggtg ttcgctacgc agttcatgca
      241 attgcgccag cagcaggtcg cgatcacgca gcatatggct gccgcgcggc accaggccgc
      301 tgctgtgatg atgatgatga tggctgctgc ccatggtata tctccttctt aaagttaaac
      361 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt
      421 aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg
      481 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
      541 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
      601 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
      661 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
      721 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
      781 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
      841 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      901 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      961 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     1021 gccctg
//
