LOCUS       sequence-139365-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein with 
            N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole 
            ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK540
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             889..897
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgctgcgag atcgcgattt actgcttgcc
      121 caactgcatg aactccggag tgaacacaga gatctggata cggttatcaa ccgcatggcg
      181 catgaaacta cttttattga ccaactctat ctccagcggc tgaaaaaacg taaacttctg
      241 ctaaaagatc agattaccaa ggttgaaagc cttctgatcc ctgatgacat tgcctgaacc
      301 tctccgttac agactttaaa aaaccggata gatacccacc gtgagcgaaa ccgcccccct
      361 gccctctgct tccagtgctt tagaagataa agcagcctct gcccccgttg tgggcattat
      421 catgggcagc cagtcagact gggaaaccat gcgccatgca gacgcattgc tgacagagct
      481 ggaaattccg catgaaaccc tcattgtttc agcacatcgt acgccagacc ggttggcgga
      541 ttatgcgcgt acggcagccg aacgcggcct gaatgtgatt attgctgggg ccggtggagc
      601 tgcacatctg ccgggtatgt gtgcggcatg gacacgcctg cccgtgctgg gtgttccagt
      661 agaatctcgc gctcttaaag gcatggacag cctgctttcc attgtgcaga tgcccggcgg
      721 cgtgcctgtg ggcactttgg ctattggggc atccggcgcc aaaaatgccg ccttgctggc
      781 cgcctccatt cttgcactgt ataacccggc ccttgccgca cgccttgaaa catggcgcgc
      841 attgcagacg gcctctgtcc ccaactctcc cattaccgaa gataaatgac ctccttacat
      901 tctccgctta agcccaatgc cgttattggc attgtggggg gtggccagct tggccgcatg
      961 tctgctgttg ctgccgcgcg ccttggtttt cgcacgcata ttcttacgcc agaggccaac
     1021 ggcccggcgg ctcaggt
//
