LOCUS       sequence-139366-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein with 
            N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole 
            ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK540
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcttaa ctggcagatt cttcatcttc cacagcggcc
      121 gttttggtgg ccaatgggcc caaggcatcc tgaataccca aattgcctgg cagcgcaccg
      181 taggggaaca gaatatttac atgccccatt ttacggccca ctctggcttc tgccttgcca
      241 tatagatgcg gaataaagcc ctgcgtggcc ataatctgcg gcagcagcgc catatcttcc
      301 ggcccgacca gatttttcat gattgcatca gaatggcgca cagcgtgcgg caaaggcagc
      361 cccgcaactg cacggatatg catgtcaaat tggtcctgcg ggcaggcatt catggtccaa
      421 tggccagaat tatgggggcg cggggccatt tcattcacca gcaggttgcc ggtgcaatca
      481 acaaacattt caacgcccag aataccaacc agctccagct tttctgcaat ggtggtggct
      541 agttttatgg cctgcgtggc aatatctggg tcaatggggg cgggggctaa tgttagatcc
      601 agaataccgt tccagtgccg gttcagcgcc gggtcaaagc accttaccgt gccatctgca
      661 ccacgcgcca ccataacact aatctcgcaa gcaaaatcca ccataccttc tgcaaccaga
      721 ggatgtggtg ccagagcgtt gaaagcctct tccagatcat ggatgtgatg cacacggcgc
      781 tggcctttgc catcatatcc attgcgagtg gttttgagaa tcagcggcaa acccactttt
      841 tctgcggctt cgtgtagcgt tttgacatcg gtaacaggat gccatggcgc gagtggaata
      901 cccgcagaag caaaaaaagt tttttctgcc aatcggtcct gactgatgcg cagaatatgc
      961 cccgaaggat gaacagggca gtgtttggct aggcggtcca gtgcatctgc gctgatgttt
     1021 tcaaattcaa aggtaacaac atcaacagcg cgggcaa
//
