LOCUS       sequence-139372-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E. coli HpaBC in monocistronic form from ePathBrick 
            vector, pETM6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Jones JA, Collins SM, Lachance DM, Vernacchio VR, Koffas MA
  TITLE     ""Optimization of naringenin and p-coumaric acid hydroxylation using
            the native E. coli hydroxylase complex, HpaBC"".
  JOURNAL   Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.
  PUBMED    26488898
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Prog. 2015 Oct 21. doi: 10.1002/btpr.2185."
COMMENT     Sequence Label: pETM6-HpaBC
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagcctag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg aaaccagaag atttccgcgc cagtacccaa cgtcctttca
      181 ccggggaaga gtatctgaaa agcctgcagg atggtcgcga gatctatatc tatggcgagc
      241 gagtgaaaga cgtcaccact catccggcat ttcgtaatgc ggcagcgtct gttgcccagc
      301 tgtacgacgc actgcacaaa ccggagatgc aggactctct gtgttggaac accgacaccg
      361 gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccgac gacctgcgcc
      421 agcaacgcga cgccatcgct gagtggtcac gcctgagcta tggctggatg ggccgtaccc
      481 cagactacaa agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt
      541 tcgagcagaa cgcccgtaac tggtacaccc gtattcagga aactggcctc tactttaacc
      601 acgcgattgt taacccaccg atcgatcgtc atttgccgac cgataaagtg aaagacgttt
      661 acatcaagct ggaaaaagag actgacgccg ggattatcgt cagcggtgcg aaagtggttg
      721 ccaccaactc ggcgctgact cactacaaca tgattggctt cggctcggca caagtgatgg
//
