LOCUS       sequence-139373-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E. coli HpaBC in monocistronic form from ePathBrick 
            vector, pETM6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Jones JA, Collins SM, Lachance DM, Vernacchio VR, Koffas MA
  TITLE     ""Optimization of naringenin and p-coumaric acid hydroxylation using
            the native E. coli hydroxylase complex, HpaBC"".
  JOURNAL   Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.
  PUBMED    26488898
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Prog. 2015 Oct 21. doi: 10.1002/btpr.2185."
COMMENT     Sequence Label: pETM6-HpaBC
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..59
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     RBS             614..636
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    651..675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(675..694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(676..694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt
       61 ggcagcagtt taggttaatt aagctgcgac tagttaaatc gcagcttcca tttccagcat
      121 caccggatgg aaacggcgtt taaagtagat aagtccatga ccttctgcac tgaggatgat
      181 gtttttaatc tccaccagat acaccagatg tgtgccaatt gcctgcacat cgcggatctc
      241 accttcaaga ctggccagcg aaccttttag caccggctgc gccagcggac ctttttgcca
      301 gcatgagagg ctaaaacgct cttccatcgc catgcctgtc atgcccgcga agtggcgtgc
      361 catcagttcc tgctcatggt tgaggacgtt gacgcacaac ttgccgttgc cctgaaaaac
      421 cgggttcatc gcactgttgg cgttaatgca caccatcagc gacggtggtg tatccgtgac
      481 cgagcagacg gccgttgccg taatcccgca ttgtccggcg tcgccctcgg tggtgataat
      541 atttaccgct gccgacaggc tggccatcgc gtcacgaaag cgcaggcgtt gttcatctaa
      601 ttgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      661 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg
      721 atcctagcaa
//
