LOCUS       sequence-139375-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK286 (mAID-NeoR)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..42
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     134..153
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(452..471)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctgggtacc
       61 ggatccaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
      121 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
      181 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
      241 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
      301 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
      361 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
      421 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
      481 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
      541 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
      601 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
      661 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
      721 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
      781 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
      841 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
      901 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
      961 cattcagggc accggacagg tcggtcttga caaaaa
//
