LOCUS       sequence-139377-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK286 (mAID-NeoR)
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..320
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      171..306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(409..428)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
       61 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      121 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      241 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
      301 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
      361 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      421 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      481 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
      541 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      601 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      661 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      721 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      781 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      841 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
      901 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
      961 ttt
//
