LOCUS       sequence-139380-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag V5( not detected by anti 
            GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Looger Lab
  TITLE     Looger Lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGmRuby2smFPV5
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             154..195
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             205..246
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             256..297
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
       61 attttggcaa agaattcggt accgcgggcc cggcgcgcca gctggatatc tccggacccg
      121 ggctagcgca attgcggccg cgccgccacc atgggcaaac ctattcctaa ccctttgctt
      181 ggactggatt caactcagca acagggcaag cctatcccca atccccttct tgggctggac
      241 tcaactcagc aacagggaaa acccatccca aatccactgt tgggtctgga ttccactggg
      301 cagggcaatt ctctgatcaa ggagaacatg aggatgaaag tggttatgga agggtctgta
      361 aatgggcatc agttcaagtg tacgggggag ggcgaaggca atccctacat ggggacccag
      421 accatgagga ttaaagtgat tgaagggggc cctctgcctt ttgcgttcga tattctggct
      481 acgtcatttg gcggcggtag tcgcacgttc atcaaatacc ccaagggtat acccgacttt
      541 ttcaaacaga gctttcctga aggttttacc tgggagcgag taacccggta tgaagatgga
      601 ggggtagtga cggtgatgca ggataccagc cttgaagatg ggtgcctcgt ctaccacgtg
      661 caagtgagag gcgtcaactt tcctagcaac ggcccagtga tgcagaagaa gacaa
//
