LOCUS       sequence-139385-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK691
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             455..472
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(525..543)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      539..586
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      623..1003
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(710..729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     920..941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgatgatac gtgagcggat agaagaaaaa
      121 ttaagggcgg cgttccaacc cgtattcctc gaagtagtgg atgaaagcta tcgtcacaat
      181 gtcccagccg gctctgaaag ccattttaaa gttgtgctgg tcagcgatcg ttttacgggt
      241 gaacgttttc tgaatcgtca tcgaatgatt tacagtactt tagcggagga actctctact
      301 accgttcatg cgctggctct gcatacttac actattaagg agtgggaagg gttgcaggac
      361 accgtctttg cctctcctcc ctgtcgtgga gcaggaagca tcgcgtaagg atccgaattc
      421 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      481 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      541 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      601 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      661 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      721 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      781 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      841 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
      901 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
      961 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg g
//
