LOCUS       sequence-139388-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The lentiviral sgGOT1 vector was generated via ligation of 
            hybridized oligos (below) into lentiCRISPR-v1 vector linearized with
            BsmBI using Gibson assembly (NEB)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
  TITLE     An Essential Role of the Mitochondrial Electron Transport Chain in 
            Cell Proliferation Is to Enable Aspartate Synthesis.
  JOURNAL   Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
  PUBMED    26232224
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "540-51"
COMMENT     Sequence Label: plentiCRISPR-sgGOT1
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        130..205
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    253..370
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgcagtttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
       61 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg ataggctgag
      121 tcaaagaagg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      181 tgaaaaagtg gcaccgagtc ggtgcttttt tgaattctag atcttgagac aaatggcagt
      241 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      301 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      361 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgagtg
      421 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      481 gaggggtcgg caattgaacc ggtgcctaga
//
