LOCUS       sequence-139390-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr 
            mutation, N-terminal His6-tag (H6EcIscS-A260T).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK693
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaaattac cgatttatct cgactactcc
      121 gcaaccacgc cggtggaccc gcgtgttgcc gagaaaatga tgcagtttat gacgatggac
      181 ggaacctttg gtaacccggc ctcccgttct caccgtttcg gctggcaggc tgaagaagcg
      241 gtagatatcg cccgtaatca gattgccgat ctggtcggcg ctgatccgcg tgaaatcgtc
      301 tttacctctg gtgcaaccga atctgacaac ctggcgatca aaggtgcagc caacttttat
      361 cagaaaaaag gcaagcacat catcaccagc aaaaccgaac acaaagcggt actggatacc
      421 tgccgtcagc tggagcgcga aggttttgaa gtcacctacc tggcaccgca gcgtaacggc
      481 attatcgacc tgaaagaact tgaagcagcg atgcgtgacg acaccatcct cgtgtccatc
      541 atgcacgtaa ataacgaaat cggcgtggtg caggatatcg cggctatcgg cgaaatgtgc
      601 cgtgctcgtg gcattatcta tcacgttgat gcaacccaga gcgtgggtaa actgcctatc
      661 gacctgagcc agttgaaagt tgacctgatg tctttctccg gtcacaaaat ctatggcccg
      721 aaaggtatcg gtgcgctgta tgtacgtcgt aaaccgcgcg tacgcatcga agcgcaaatg
      781 cacggcggcg gtcacgagcg cggtatgcgt tccggcactc tgcctgttca ccagatcgtc
      841 ggaatgggcg aggcctatcg catcacaaaa gaagagatgg cgaccgagat ggaacgtctg
      901 cgcggcctgc gtaaccgtct gtggaacggc atcaaagata tcgaagaagt ttacctgaac
      961 ggtgacctgg aacacggtgc gccgaacatt ctcaacgtca gcttcaacta cgttgaaggt
     1021 gagtc
//
