LOCUS       sequence-139391-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr 
            mutation, N-terminal His6-tag (H6EcIscS-A260T).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK693
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tccttaatga tgagcccatt cgatgctgtt
      121 cagatccacg ccctgcttgt acatttccca cagcggagaa aggtcacgca gacgaccgat
      181 ggatttacga actaactcga tggtgtagtc gatctcttct tcagtagtaa aacgacctaa
      241 agagaaacgg atagagctat gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta
      301 ggacggttcg aggcttgctg acgtacaggc ggaacctgaa gaaactgcga ggtctttcag
      361 cgccataatc agcgactcac cttcaacgta gttgaagctg acgttgagaa tgttcggcgc
      421 accgtgttcc aggtcaccgt tcaggtaaac ttcttcgata tctttgatgc cgttccacag
      481 acggttacgc aggccgcgca gacgttccat ctcggtcgcc atctcttctt ttgtgatgcg
      541 ataggcctcg cccattccga cgatctggtg aacaggcaga gtgccggaac gcataccgcg
      601 ctcgtgaccg ccgccgtgca tttgcgcttc gatgcgtacg cgcggtttac gacgtacata
      661 cagcgcaccg atacctttcg ggccatagat tttgtgaccg gagaaagaca tcaggtcaac
      721 tttcaactgg ctcaggtcga taggcagttt acccacgctc tgggttgcat caacgtgata
      781 gataatgcca cgagcacggc acatttcgcc gatagccgcg atatcctgca ccacgccgat
      841 ttcgttattt acgtgcatga tggacacgag gatggtgtcg tcacgcatcg ctgcttcaag
      901 ttctttcagg tcgataatgc cgttacgctg cggtgccagg taggtgactt caaaaccttc
      961 gcgctccagc tgacggcagg tatccagtac cgctttgtgt tcggttttgc tggtgatgat
     1021 gtgcttgcct tttttctga
//
