LOCUS       sequence-139392-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The lentiviral sgGOT1 vector was generated via ligation of 
            hybridized oligos (below) into lentiCRISPR-v1 vector linearized with
            BsmBI using Gibson assembly (NEB)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
  TITLE     An Essential Role of the Mitochondrial Electron Transport Chain in 
            Cell Proliferation Is to Enable Aspartate Synthesis.
  JOURNAL   Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
  PUBMED    26232224
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "540-51"
COMMENT     Sequence Label: plentiCRISPR-sgGOT1
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(256..276)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(283..306)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      306..315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(335..546)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ccggttcttc cgtctggtgt atcttcttct ggcggttctc ttcagccggg tggcctcggc
       61 tgtttcgccg ctgtcgaaca gcagggctcc gatcaggttc ttcttgatgc tgtgccggtc
      121 ggtgttgccc agcaccttga atttcttgct gggcaccttg tactcgtcgg tgatcacggc
      181 ccagcccaca gagttggtgc cgatgtccag gccgatgctg tacttcttgt cggctgctgg
      241 gactccgtgg ataccgacct tccgcttctt ctttggggcc atcttatcgt catcgtcttt
      301 gtaatccatg gtggcggatc ccgcgtcacg acacctgtgt tctggcggca aacccgttgc
      361 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
      421 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
      481 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
      541 ctgcccactg acgggcaccg gagccactcg agccggcgcc aaagtggatc tctgctgtcc
      601 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
      661 atgtctgttg ctattatgtc tactattc
//
