LOCUS       sequence-139394-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli glutamine synthetase, N-terminal His6 tag 
            and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK642
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgtccgctg aacacgtact gacgatgctg
      121 aacgagcacg aagtgaagtt tgttgatttg cgcttcaccg atactaaagg taaagaacag
      181 cacgtcacta tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt
      241 gacggctcct cgattggcgg ctggaaaggc attaacgagt ccgacatggt gctgatgcca
      301 gacgcatcca ccgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc
      361 gacatccttg aacctggcac cctgcaaggc tatgaccgtg acccgcgctc cattgcgaag
      421 cgcgccgaag attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa
      481 cctgaattct tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt
      541 gctatcgacg atatcgaagg tgcatggaac tcctccaccc aatacgaagg tggtaacaaa
      601 ggtcaccgtc cggcagtgaa aggcggttac ttcccggttc caccggtaga ctcggctcag
      661 gatattcgtt ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat
      721 caccacgaag tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc
      781 aaaaaagctg acgaaattca gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc
      841 ggtaaaaccg cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac
      901 tgccacatgt ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt
      961 ctgtct
//
