LOCUS       sequence-139395-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli glutamine synthetase, N-terminal His6 tag 
            and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK642
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttagacg
       61 ctgtagtaca gctcaaactc taccggatgc ggagtcatac gcacgcggtc atcttcttcg
      121 cgacgcagag cgatgtacgc atcaattgct tcgtcagtga acacgccacc ggctttcagg
      181 aactcgcggt ccagatccag ttcgttcagt gcttcttcca gagagcctgc aacctgtggg
      241 atctctttcg cttcttctgg cggcaggtca aacaggtttt tgtccatggc ttcgcccgga
      301 tggatcttgt tcttgatacc atcaagaccg gccatcagca gggcagcaaa gcacaggtac
      361 gggttagctg ccggatccgg gaaacgtact tcgatacgac gtgctttcgg agaagaaacc
      421 accggaatac ggatagacgc agaacggtta cgcgcagagt aagccagcat taccggtgct
      481 tcatagcccg ggaccagacg cttataagag ttggtggtcg ggtttgccag ggcgttaatc
      541 gctttagcgt gtttgattac gccgccaatg tagtacagcg cctgctcaga cagacctgcg
      601 tatttgtcgc ctgcgaacag gttaacgccg tttttagaca gagacatgtg gcagtgcata
      661 ccggagccgt tatcaccgaa catcggtttt ggcataaagg tcgcggtttt accgaagcgg
      721 tgcgctacgt tgtgcacaac atatttgtag atctgaattt cgtcagcttt tttggtcatg
      781 gtattgaagc gggtagccac ttcgttctga ccagcagtcg ctacttcgtg gtgatgggct
      841 tcaaccacca gacccatctg ttccatcacc agacacattt cagaacgaat atcctgagcc
      901 gagtctaccg gtggaaccgg gaagtaaccg cctttcactg ccggacggtg acctttgtta
      961 ccaccttcgt attgg
//
