LOCUS       sequence-139401-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT),
            synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK683
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             362..370
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagttacc attccaattc
       61 aactgggtac ttaccagtca aacaagcagt acaatgaccg gacttttcga aacattccaa
      121 accgttacca ttttcttgga tcatgatgtc gtgcttcttt tccttttgct tcttgaactt
      181 gataccttct tgaacagaag aaaccaaacc ttcaacagat aagtaaacaa cggagttagc
      241 acctaagtat tcagctaagt gatcgaactc tggtttgttg gcgattaatt cctccttagt
      301 tggaatgttg atacccatga aacatgggta cttgattggt ggagaagcaa ctctaatatg
      361 gacctcctta gcaccagatt ccttcaacaa cttaataatt ggagagatgg tgttaccacg
      421 aacaatggaa tcgtcgacta aaacaattct cttacccttg aagttgtcgg acaaaacacc
      481 gaacttttta gcgataccca attgacgcaa tctcatattt ggttgaatga aagttctacc
      541 aacatatctg ttcttacaca aaacttcgac gtatggcaaa ccacacttac cagcgtaagc
      601 taaagcggct ggggtagcgg attcagggac agtagaaacc aagtcagcgt cgactggagc
      661 ttcgatagcc aattgttgac cacatctgta tctgacggta taaaccattt gatcttcgaa
      721 catggagtca ggtctggcaa agtaaacgta ttcgaagatg cagaaagcga ctgggttacc
      781 ttcagatctg gagatgatgt ccaaagtttg aacgttgtgt ctagaaattt caacgatttc
      841 accaggcaaa acttctctat aatatctggc accaatagat aaaaaagagc aagattcgga
      901 agaaacaacc caaccttcag tttcagaggt ctttttttcc ttgtcgttaa tgtcggaaac
      961 aggaatcaat ctaccgatac ataatggtct gttaccgtat ggatctctaa cagcgtagat
     1021 gacatcacgg tgcataatca acaaggagta agcagttggg gcttccttca ttaagttctt
     1081 gatacgggcg acccagtctg gg
//
