LOCUS       sequence-139405-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal double-FLAG tag, Puromycin-resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK25
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             35..58
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(68..101)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggattataaa gatgatgatg ataaatccgg agccgattat aaagatgatg atgataaatg
       61 aactagtgaa gttcctatac tttctagaga ataggaactt caaataaaca tatgggcgcg
      121 ccctgttgga atatactatt caacctacaa aaataacgtt aaacaacact actttatatt
      181 tgatatgaat ggccacacct tttatgccat aaaacatatt gtaagagaat accactcttt
      241 ttattccttc tttccttctt gtacgttttt tgctgtgagt aggtcgtggt gctggtgttg
      301 cagttgaaat aacttaaaat ataaatcata aaactcaaac ataaacttga ctatttattt
      361 atttattaag aaaggaggta ccggtggcgc gccatgaccg agtacaagcc tacagtgcga
      421 ctggccacac gcgatgacgt tcccagagcc gttcggacgt tggctgccgc gtttgcggac
      481 tatcccgcaa ctcgccatac tgtggaccct gatcgccaca tcgagagggt gactgagctt
      541 caggagctgt tcctgaccag ggtagggctg gacataggca aggtctgggt ggccgacgat
      601 ggagccgctg ttgcggtgtg gactacccca gagagcgtgg aagcaggggc tgtctttgcc
      661 gaaattgggc caaggatggc cgaactgtct gggtcccgtt tggctgccca acagcagatg
      721 gaaggactgc ttgccccaca tcgacccaag gagcctgcat ggttcctcgc taccgtgggt
      781 gtgagcccag atcaccaggg caaaggtctg ggatcagccg tcgttctgcc tggcgtcgag
      841 gctgctgaga gagcaggagt accggcattc ctcgaaacca gtgctccacg gaacctcc
//
