LOCUS       sequence-139407-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with 
            C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) 
            with C-terminal c-myc epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK371
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(105..560)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(242..263)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     454..473
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(639..804)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     725..749
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(952..975)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac cctatgcggt
       61 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
      121 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      181 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
      241 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      301 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
      361 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
      421 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
      481 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
      541 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      601 tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg
      661 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac
      721 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa
      781 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat
      841 ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat
      901 tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc
      961 gtcatccttg taatccatcg atactagtgc gaatttattg taccaatata cacccttgga
     1021 ctccctacct ttttcaggct cgatgaagat gcattccttg gcctctctcc
//
