LOCUS       sequence-139418-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2227
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(56..89)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(149..346)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 actcactata gggcgaattg ggtgcatagg ccactagtgg atctgatatc acctaataac
       61 ttcgtatagc atacattata cgaagttata ttaagggttc tcgagagctc gtttaaactg
      121 gatggcggcg ttagtatcga atcgacagca gtatagcgac cagcattcac atacgattga
      181 cgcatgatat tactttctgc gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa
      241 aaaaaatata aatcacgcta acatttgatt aaaatagaac aactacaata taaaaaaact
      301 atacaaatga caagttcttg aaaacaagaa tctttttatt gtcagtactc tttacaacac
      361 tcccttcgtg cttgggactt cagaacttcc agtaagacta gtagccgcgc gcatggcaac
      421 agccagagat ttaaaagcgc tttcagcacg atgatggtca ttactaccat ataagcaggt
      481 aacatgcaaa gtaattccag ctgctaccga aaaggaatat agtaagtgag ggatcatttc
      541 acaggacaat tccccaacct tttcacgctt taatcccaaa tcgataacag catagggccg
      601 tcccgacaag tcaaccacgc ttctagaaag agcttcgtca agtggacaat aagcatgtcc
      661 aaatctttta acgccggcaa agttacccat agcctgcttg aatgcaatac caagtgcaat
      721 agcagtatct tctgcagtgt gatgatcatc gatgattaaa tcacctcttg agtaaagtcg
      781 taagctccag cctgcatgtt tagccagtgc atgatacatg tgatccaaga at
//
