LOCUS       sequence-139423-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The lentiviral sgMDH1 vectors was generated via ligation of 
            hybridized oligos (below) into lentiCRISPR-v1 vector linearized with
            BsmBI using Gibson assembly (NEB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
  TITLE     An Essential Role of the Mitochondrial Electron Transport Chain in 
            Cell Proliferation Is to Enable Aspartate Synthesis.
  JOURNAL   Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
  PUBMED    26232224
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "540-51"
COMMENT     Sequence Label: plentiCRISPR-sgMDH1
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..155
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        235..310
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    358..475
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        544..755
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      775..784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             784..807
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             814..834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcatcgatc aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
       61 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      121 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      181 gctttatata tcttgtggaa aggacgaaac accggacatc tggatactga gtcggtttta
      241 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      301 gagtcggtgc ttttttgaat tctagatctt gagacaaatg gcagtattca tccacaattt
      361 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      421 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      481 ttattacagg gacagcagag atccactttg gcgccggctc gagtggctcc ggtgcccgtc
      541 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
      601 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
      661 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
      721 ttctttttcg caacgggttt gccgccagaa cacaggtgtc gtgacgcggg atccgccacc
      781 atggattaca aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc
      841 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct
      901 gtgggctggg ccgt
//
