LOCUS       sequence-139426-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The lentiviral sgMDH1 vectors was generated via ligation of 
            hybridized oligos (below) into lentiCRISPR-v1 vector linearized with
            BsmBI using Gibson assembly (NEB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
  TITLE     An Essential Role of the Mitochondrial Electron Transport Chain in 
            Cell Proliferation Is to Enable Aspartate Synthesis.
  JOURNAL   Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
  PUBMED    26232224
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "540-51"
COMMENT     Sequence Label: plentiCRISPR-sgMDH1
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(257..277)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(284..307)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      307..316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(336..547)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    616..733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccggttctt ccgtctggtg tatcttcttc tggcggttct cttcagccgg gtggcctcgg
       61 ctgtttcgcc gctgtcgaac agcagggctc cgatcaggtt cttcttgatg ctgtgccggt
      121 cggtgttgcc cagcaccttg aatttcttgc tgggcacctt gtactcgtcg gtgatcacgg
      181 cccagcccac agagttggtg ccgatgtcca ggccgatgct gtacttcttg tcggctgctg
      241 ggactccgtg gataccgacc ttccgcttct tctttggggc catcttatcg tcatcgtctt
      301 tgtaatccat ggtggcggat cccgcgtcac gacacctgtg ttctggcggc aaacccgttg
      361 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
      421 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
      481 gcaccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
      541 tctgcccact gacgggcacc ggagccactc gagccggcgc caaagtggat ctctgctgtc
      601 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
      661 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
      721 ttttctttta aaattgtgga tgaatactgc catttgtctc aagatctaga attcaaaaaa
      781 gcaccgactc ggtgcca
//
