LOCUS       sequence-139427-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STAG2 cDNA (CCDS43990) V181M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Kim JS, He X, Orr B, Wutz G, Hill V, Peters JM, Compton DA, Waldman 
            T
  TITLE     Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells
            Harboring Tumor-Derived Mutations in STAG2.
  JOURNAL   PLoS Genet. 2016 Feb 12;12(2):e1005865. doi: 
            10.1371/journal.pgen.1005865. eCollection 2016 Feb.
  PUBMED    26871722
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-02-12"; volume: "12(2):e1005865. doi"; pages: "
            10.1371/journal.pgen.1005865. eCollection 2016 Feb"
COMMENT     Sequence Label: pcDNA3.1-FLAG-SBP-STAG2 (V181M)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..50
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             51..164
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ccgagctcgg atccaccggt accatggact acaaggacga cgacgacaag atggacgaga
       61 agaccaccgg ctggcgcggc ggccacgtgg tggagggcct ggccggcgag ctggagcagc
      121 tgcgcgcccg cctggagcac cacccccagg gccagcgcga gcccggcggc atagcagctc
      181 cagaaatacc aactgatttt aatctactac aggagtcaga aacacatttt tcttctgaca
      241 cagattttga agatatcgaa ggaaaaaacc aaaagcaagg caaaggcaaa acttgtaaaa
      301 aaggcaaaaa gggcccagca gaaaagggca aaggtggaaa tggaggagga aaacctcctt
      361 ctggtccaaa ccgaatgaat ggtcatcacc aacagaatgg agtggaaaac atgatgttgt
      421 ttgaagttgt taaaatgggc aagagtgcta tgcagtcggt ggtagatgat tggatagaat
      481 catacaagca tgaccgagat atagcacttc ttgaccttat caactttttt attcagtgtt
      541 caggctgtaa aggagttgtc acagcagaaa tgtttagaca tatgcagaac tctgagataa
      601 ttcgaaaaat gactgaagag ttcgatgagg atagtggaga ttatccactt accatggctg
      661 gtcctcagtg gaagaagttc aaatccagtt tttgtgaatt cattggcatg ttagtacggc
      721 aatgtcaata tagtatcata tatgatgagt atatgatgga tacagtcatt tcacttctta
      781 caggattgtc tgactcacaa gtcagagcat t
//
