LOCUS       sequence-139443-        9924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector with chimeric endothelial enhancer/promoter upstream of 
            attR1/R2 cassette and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 9924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 343 pTol2-fli1epDest
FEATURES             Location/Qualifiers
     source          1..9924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    3430..3554
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3579..3584,3585..3602,3603..3609)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3663..4322
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3729..3748)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4664..4969
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4848..4867
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5010..5134)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    5179..5313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5203..5222)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5257..5276
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7454..7641,7642..7653)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7687..7703)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7687..7703)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7740..7760)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7740..7758)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7779..7795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7779..7795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7792..7814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7803..7819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7827..7833,7834..7851,7852..7857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7872..7893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8010..8027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8181..8769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8261..8280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8940..9731,9732..9800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9563..9582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9801..9905)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 taattcgccc ttggagatct catctttgac ccataaacat acactaaaac ccaacagtca
     1381 actttatcaa atgaaatgag tgtaattaac tcaaaattta ctgaatgttg attctactca
     1441 ttcgaaaaga gttttaaact cagtgttgaa ggtagtgagt tcattaaata cctcattact
     1501 tcaacttaac tggagtaagt tcatggtact cacatagatt agtttagctc aaatggtttg
     1561 tagcagtcgg ttccctcaag cagtttgagt tgccttaact ttttgggttt tacagtactc
     1621 agttggtttg agttcacttc acttattggg ttcaacatgt gctcaaattg catcgtttaa
     1681 tcaaatagat taagttcaca gtactcatta ggattagttt ttgaacttaa atggtttgtt
     1741 gcagtcggtt tcctcaaacg gtttgagttt ccataacttt atgggttttt cagtgtagta
     1801 aacatatgca tccacagatt ataatagtaa cctgcatgtt ttttccttca caatttgaca
     1861 cagatacctc acatgattga cttttaactg tacactttag tcgacaatga gaagggtgtc
     1921 tcaatctatg ggtatttcct gttccacaca atctggtttc ctgtcaggtt tccttttcct
     1981 cctgaaacaa aagacaggaa tgccctctgt ataaaatagc acagcgcttt taatgggtct
     2041 ctgtacatcc cgacctcatg acgcatcttc tgcgggacag ctcaatcacg atcaagttgg
     2101 cttatgaaaa cagttttttg tccccctgtt aagttaaaag cacggtagca tacatgcatt
     2161 attcatcatg cagcatattc agcagtacaa aagatgagct cttgacacac ctcagcgtaa
     2221 aatctctatt ccagcgtaat cctgcggttc tggatggtta agcgagcctt gcaaacacgg
     2281 ccaatttata gccatgttga aaatagcagc tttgtgagca tttcgacctc aatgtaccaa
     2341 gaatgcgtgc tcggtaactg aaaaagaggg ggtggagatt ttggatttag agatggaatc
     2401 acatgcacgc tgtatatgct atataaaaat agcaggcatt taatgcatgc aaaatatgtt
     2461 tcgacactaa acactacaag tcggaaatat atctattttt ctttttttaa cgaaatgaga
     2521 catttaatgc gcagtaaatg tagtctaaca cgcacaatat tcctattatt gtccatgaaa
     2581 aatgtgaggt crtatgctac tgatttaaag aaaatgttga acaggcacag cacacaagag
     2641 gtatcagcct ctctatatgg tccatacagc accaggctcg ctgcgttcat gtgcattcag
     2701 aggcgcgcac acgtgtggag cagacagact cgacgcagcg ggctttgctt tttattcatg
     2761 gaagcccatt ttagaagaag ctcgggcaga ctcgtcacat tcttcacttc accataggta
     2821 gatcaaatca aagttattgg atataacgga acaatattca tcatattatg ragattatta
     2881 ttaagaccga gtgcattaga catattttgt atttcgatcg ttggaaaaag tatatatttt
     2941 attttagcct taaactatgg ttagcctata ttaatcagca taatctgtta tttcagacgt
     3001 tgtattaatg attatttcta gcacagcttt gatgcatcaa tgcaatgttt ctacaaaccc
     3061 gccttgttta atgtcggaaa aaaagcgcaa caaatgtaaa gagttggcag gcattctgta
     3121 aatatttcca ggcaaaaaca tgcacaaagg aaagaaacac cattgttttg gtgtcttatc
     3181 ttcgctggac aaatatcctg ctttgggtgc cgtaatgaag gcagggccag tgacacacac
     3241 acatacacac acacacatac acgcatacac ggcttccttc tcaagcgcag tgacttcact
     3301 tcccaaattt agaggaaaaa aaaccatccg gcgaaactgt ctctgtctct ccgccacata
     3361 tcgggggctg gaattaattc agacgcgaag ggcgaattat cgctagcatc gattcgaatt
     3421 caaggatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     3481 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     3541 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     3601 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     3661 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     3721 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     3781 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     3841 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     3901 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     3961 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     4021 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     4081 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     4141 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     4201 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     4261 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     4321 aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     4381 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     4441 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     4501 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     4561 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     4621 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     4681 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     4741 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     4801 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     4861 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     4921 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     4981 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     5041 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     5101 acgtttctcg ttcagctttc ttgtacaaag tggttgatcc tctcgagcct ctagaactat
     5161 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     5221 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5281 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5341 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat
     5401 tgggccctcg agcatatgag atctgtcccg atctttctct tctgtgctgt caatttagag
     5461 tagtttttga ggtaatttgg gtgcattctc tgtaaaaaca acaaataaat atttatattg
     5521 tatgcaataa atgtaacaat tacacataca ctctctgatt acagcattaa agggttagtt
     5581 cacccaaaaa tgaaaagatg tcattaatga ctcaccctca tgtcgttcca agcccgtaac
     5641 acctccgttc atcttcggaa cacagtttaa gatattttag atttagtccg agagctttct
     5701 gtccctccac tgaaaatgta tgtacggtat actgtccatg tccagaaagg taataaaaac
     5761 atcaaagtag tccatgtgac atcagtgggt taggtagaat tatttgaagc atcgaaaata
     5821 cattttggtc caaaaataac aaaacctacg actttattca gcattgtagc gtcactgcga
     5881 agtcgtgaac gcggattgac aacagacccg gaagagaata caatgccgaa taaagtcgta
     5941 ggttttgtta tttttggacc aaaatgtatt cgatgcttca aaaaattcta actaacccac
     6001 tgatgtcaca tggactactt tgatgttttt attacctttc tggacatgga cagtataccg
     6061 tacatacatt ctcaatggag gcacagaaag ctctcggact aaatctaaaa tatcttaaac
     6121 tgtgttctga agatgaacgg aggtcttacg ggcttggaac gacatgaggg cgagtcatta
     6181 atgacattat tttcattttt gggtgaacta accctttaat aaaactgaca tttgcagcca
     6241 attgcacact ttataatgtt aaatacttgt tgattttaga aatacagtag catttagtga
     6301 caatcaaaca ttaattttat ttctgattat attgacaaaa acacaggact gtaaggctga
     6361 catgacaggg tgaattgatt tcattacatt agttttgatg gtaacacttt tacaataagg
     6421 ttcgttagtt aactacatta gttaacatga actaataatg aactgcacgt atacatgtta
     6481 atttcaacat ttactaatac tttatatctt gttaacatta gttaatgcac tgtgaactaa
     6541 catgaacaaa caatgaacat ctgtatttct attaactaac gttaacaaag attaacagat
     6601 acagtaacaa atgtattgct catggttatt taatgttagt taatacatga actaatcaac
     6661 ctcattgtaa agtgttaccg ttttgacaat acaacatgtt tggaaaaaaa tttctcgctt
     6721 ggttttcttg tgttttgcat gaattcaaac acaatgagag cacggaatga taatgattat
     6781 tcaaagctgc aataggcgcc aaatttcccg tggacctaac gttaccaatt acaaatgcat
     6841 tttagcattt tattacgtga gccttatatt gtcaatcgca tgcattagtg aaaataggcc
     6901 tactacacat ataaaaggcg cgccatcaac gtcaaaacat gcacacccaa aaaaaaaaaa
     6961 aaaaaaaaaa aaaaaaacta tcagtaaaac aaaatactta atgtacttac ctcaatatgc
     7021 ttccttaggt ttgatggcga acttttgaag gccgatattt ctttattaag cgggagacag
     7081 aggacacatt tcatcttgaa tgaatcttta tttacaccac ttaaagaaaa gaattcgcga
     7141 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     7201 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     7261 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     7321 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     7381 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     7441 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     7501 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     7561 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     7621 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     7681 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     7741 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     7801 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7861 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7921 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7981 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8041 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8101 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8161 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8221 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8281 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8341 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8401 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8461 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8521 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8581 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8641 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8701 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8761 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8821 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8881 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8941 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9001 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9061 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9121 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9181 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9241 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9301 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9361 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9421 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9481 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9541 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9601 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9661 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9721 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9781 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9841 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9901 ccgcgcacat ttccccgaaa agtg
//
